Open Access
CC BY-NC-ND 4.0 · Endosc Int Open 2019; 07(05): E701-E707
DOI: 10.1055/a-0832-8283
Original article
Owner and Copyright © Georg Thieme Verlag KG 2019

CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients

Jasper L.A. Vleugels*
1   Department of Gastroenterology and Hepatology, Amsterdam University Medical Center, location Academic Medical Center, Amsterdam, the Netherlands
,
Sanne M. van Neerven*
2   Cancer Center Amsterdam, Laboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental and Molecular Medicine (CEMM), Amsterdam University Medical Center, location Academic Medical Center, Amsterdam, the Netherlands
,
Monique E. van Leerdam
3   Department of Gastroenterology and Hepatology, Netherlands Cancer Institute, Amsterdam, the Netherlands
,
Linda K. Wanders
1   Department of Gastroenterology and Hepatology, Amsterdam University Medical Center, location Academic Medical Center, Amsterdam, the Netherlands
,
Meike de Wit
4   Department of Pathology, Netherlands Cancer Institute, Amsterdam, the Netherlands
,
Beatriz Carvalho
4   Department of Pathology, Netherlands Cancer Institute, Amsterdam, the Netherlands
,
Pien M. Delis-van Diemen
4   Department of Pathology, Netherlands Cancer Institute, Amsterdam, the Netherlands
,
Frank G.J. Kallenberg
1   Department of Gastroenterology and Hepatology, Amsterdam University Medical Center, location Academic Medical Center, Amsterdam, the Netherlands
,
Louis Vermeulen
2   Cancer Center Amsterdam, Laboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental and Molecular Medicine (CEMM), Amsterdam University Medical Center, location Academic Medical Center, Amsterdam, the Netherlands
,
Jeroen A. Beliën
4   Department of Pathology, Netherlands Cancer Institute, Amsterdam, the Netherlands
5   Department of Pathology, Amsterdam University Medical Center, location VU University Medical Centre, Amsterdam, the Netherlands
,
James E. East
6   Translational Gastroenterology Unit, Nuffield Department of Medicine, University of Oxford, United Kingdom
,
Gerrit A. Meijer
4   Department of Pathology, Netherlands Cancer Institute, Amsterdam, the Netherlands
,
Evelien Dekker
1   Department of Gastroenterology and Hepatology, Amsterdam University Medical Center, location Academic Medical Center, Amsterdam, the Netherlands
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Weitere Informationen

Publikationsverlauf

submitted 25. September 2018

accepted after revision 04. Dezember 2018

Publikationsdatum:
08. Mai 2019 (online)

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Abstract

Background and study aims Microsatellite instability accelerates colorectal cancer development in patients with Lynch syndrome (LS). Previous research showed that virtual chromoendoscopy increases detection of adenomas during colonoscopy surveillance of patients with LS. Because previous research revealed that Lynch patients have an increased vascular network in the oral mucosa, we hypothesized that increased vascularization of LS-associated adenomas is the cause of better detection with virtual chromoendoscopy.

Patients and methods In this pilot study, patients with LS having a proven germline mutation were selected from two tertiary referral hospitals and non-LS patients from an outpatient colonoscopy center. Adenomas from patients with LS were exactly matched in size and histology with adenomas from non-LS patients. Initial adenoma diagnosis was confirmed by a specialist pathologist. All adenomas were stained with CD31 and adenomatous tissue was annotated by the specialist pathologist. Image analysis of CD31-positive microvessel density was conducted using FIJI software.

Results Colonoscopy of 63 patients with LS and 24 non-LS patients provided 40 adenomas that could be exactly matched in size and histology. In image-analysis, the CD31-positive microvessel density (2.49 % vs. 2.47 %, P = 0.96), the average size of CD31-positive structures (514 μm2 vs. 523 μm2, P = 0.26) nor the amount of vascular structures per mm2 (183 vs. 176, P = 0.50) differed between adenomas of LS patients and non-Lynch patients.

Conclusion The outcomes of this pilot case-control study did not provide further insights into the mechanism of increased adenoma detection in LS patients using virtual chromoendoscopy techniques.

* These authors contributed equally.