Total Flavonoids of Bidens pilosa Ameliorates Bone Destruction in Collagen-Induced Arthritis

Mengqin Hong; Xingyu Fan; Shengxiang Liang; Wang Xiang; Liting Chen; Yuzhong Yang; Yueyi Deng; Min Yang

doi:10.1055/a-1352-5124

Planta Medica, Inhaltsverzeichnis

Planta Med 2021; 87(07): 550-559
DOI: 10.1055/a-1352-5124

Biological and Pharmacological Activity

Original Papers

Total Flavonoids of Bidens pilosa Ameliorates Bone Destruction in Collagen-Induced Arthritis

Mengqin Hong‡

¹Department of Rheumatology and Immunology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China

,

Xingyu Fan‡

¹Department of Rheumatology and Immunology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China

,

Shengxiang Liang

²Department of Social Medicine and Health Service Management, Army Medical University (Third Military Medical University), Chongqing, China

,

Wang Xiang

¹Department of Rheumatology and Immunology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China

,

Liting Chen

¹Department of Rheumatology and Immunology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China

,

Yuzhong Yang

³Department of Pathology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China

,

Yueyi Deng

⁴School of Pharmacy, Guilin Medical University, Guilin, Guangxi, China

,

Min Yang

¹Department of Rheumatology and Immunology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China

› Institutsangaben

Abstract

Rheumatoid arthritis is a chronic autoimmune disease characterized by the infiltration of synovial inflammatory cells and progressive joint destruction. Total flavonoids of Bidens pilosa have been used against inflammation in rheumatoid arthritis, but its role in bone destruction remains to be explored. The aim of this paper was to study whether total flavonoids of B. pilosa relieve the severity of collagen-induced arthritis in rats, particularly whether it regulates the production of proinflammatory cytokines and the receptor activator of nuclear factor-κB/receptor activator of nuclear factor-κB ligand/osteoprotegerin signaling pathway. In this research, a collagen-induced disease model was induced in adult rats by subcutaneous injection of collagen II. Total flavonoids of B. pilosa at different doses (40, 80, and 160 mg/kg/d) were administered intragastrically, while methotrexate (1 mg/kg/w) was injected intraperitoneally as a positive control. Paw swelling, arthritis score, and body weight were assessed and evaluated. The severity of joint damage was determined using X-ray and confirmed by histopathology. The expression levels of receptor activator of nuclear factor-κB ligand, osteoprotegerin, IL-1β, IL-17, and TNF in the serum and tissue were assayed using ELISA and immunohistochemistry. We found that total flavonoids of B. pilosa attenuated collagen-induced arthritis at the macroscopic level, and total flavonoids of B. pilosa-treated rats showed reduced paw swelling, arthritis scores, and X-ray appearance of collagen-induced arthritis in addition to improved histopathological results. These findings were consistent with reduced serum and tissue receptor activator of nuclear factor-κB ligand, TNF, IL-1β, and IL-17 levels but increased osteoprotegerin levels. Our data suggest that total flavonoids of B. pilosa attenuate collagen-induced arthritis by suppressing the receptor activator of nuclear factor-κB ligand/receptor activator of nuclear factor-κB/osteoprotegerin pathway and the subsequent production of proinflammatory cytokines. In addition, total flavonoids of B. pilosa may be a promising therapeutic candidate for the management of rheumatoid arthritis.

Key words

Bidens pilosa - Asteraceae - rheumatoid arthritis - collagen-induced - arthritis - osteoprotegerin - RANKL

Volltext

Referenzen

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