Planta Med 2022; 88(08): 628-638 DOI: 10.1055/a-1528-1760
Biological and Pharmacological Activity
Original Papers
Suffrutines A and B Inhibit the Expression of Inflammatory Mediators in LPS-Induced RAW264.7 Cells by Suppressing the NF-κB Signaling Pathway
Chun Chen
1
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
,
Ze-Feng Zhu
1
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
2
Department of Pharmacy, the Fifth Affiliated Hospital of Jinan University, Heyuan, Guangdong, China
,
Wen-Xing Nie
1
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
,
Yong Zou
1
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
3
Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Guangzhou, Guangdong, China
› InstitutsangabenGefördert durch:
The National Natural Science Foundation of China
21272280
Gefördert durch:
The National Key Research and Development Project of China
2016YFA0602900
Gefördert durch:
The Key Project of Guangdong Natural Science Foundation
2016A030311033
Gefördert durch:
STPGC
201604020125
Gefördert durch:
Basic and Applied Basic Research Foundation of Guangdong Province
Appl: 011249461088
Gefördert durch:
Guangdong Provincial Key Laboratory of Construction Foundation
2019B030301005
Flueggea suffruticosa is a traditional Chinese medicine that has been commonly used for the treatment of inflammatory ailments, including rheumatism and lumbago. Suffrutines A and suffrutines B are a pair of novel E,E and Z,E isomeric indolizidine alkaloids isolated from the roots of F. suffruticosa. However, their anti-inflammatory activity has not been reported thus far. The aim of this study was to investigate the inhibitory effect of inflammatory mediators and possible mechanisms of suffrutines A and B in lipopolysaccharide-induced RAW264.7 cells. Results showed that suffrutines A and B could remarkably inhibit the production of nitric oxide, prostaglandin E2, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 in lipopolysaccharide-induced RAW264.7 cells. Further evaluation demonstrated that compared with suffrutines A, suffrutines B could more significantly inhibit the phosphorylation of IKKα/β, the
degradation of IκBα, and the nuclear translocation of the p65 and p52 subunits in the canonical and non-canonical nuclear factor-κB pathways. Therefore, suffrutines B exhibited more potent inhibitory activity on inflammatory mediators than suffrutines A.
Key words
Euphorbiaceae -
Flueggea suffruticosa
-
inflammatory mediators -
NF-κB signaling pathway -
RAW264.7 -
suffrutines A -
suffrutines B
Supporting Information
A series of NMR spectra and single-crystal X-ray crystallography of SA and SB are available as Supporting Information.
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