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Horm Metab Res 2021; 53(10): 692-698
DOI: 10.1055/a-1587-9211
Endocrine Research

Clinical Value and Role of miR-129-5p in Non-Alcoholic Fatty Liver Disease

Yuan Wang‡
1   Sub-Health Clinic of Physical Examination Center, Beijing Fengtai Hospital of Integrated Traditional Chinese and Western Medicine, Beijing, China
,
Yujia Feng‡
2   Department of Gastroenterology, People's Hospital of Rizhao, Rizhao, China
,
Han Zhang
3   Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, China
,
Qinghui Niu 
3   Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, China
,
Kun Liang
4   Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, China
,
Cheng Bian
4   Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, China
,
Hong Li‡
5   Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital,Tongji University School of Medicine, Shanghai, China
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Abstract

This study was to evaluate the expression of miR-129–5p in non-alcoholic fatty liver (NAFLD) patients and its clinical value and explore its regulatory effect on insulin resistance (IR). A total of 117 NAFLD patients and 110 healthy controls were included. The levels of miR-129-5p were detected by qRT-PCR. To assess the diagnostic value of miR-129-5p for NAFLD, the receiver operating characteristic curve (ROC) was established. C57Bl/6 mice were supplied with high-fat diet to establish NAFLD model. Intraperitoneal insulin tolerance test (IPITT) was carried out to evaluate the effect of miR-129-5p on IR in NAFLD animal model. miR-129-5p was highly expressed in the serum of NAFLD patients, and patients with HOMA-IR ≥2.5 had higher level of miR-129-5p than those with HOMA-IR <2.5. miR-129-5p had the ability to differentiate NAFLD patients from healthy individuals and might be associated with the development of IR. Serum miR-129-5p was positively correlated with the levels of HOMA-IR, BMI, total cholesterol (TC), and triglyceride (TG) in NAFLD patients. Downregulation of miR-129-5p regulates lipid metabolism and insulin sensitivity in NAFLD mice model. MiR-129-5p was upregulated in NAFLD patients and might be a potential diagnostic biomarker. The regulatory effect of miR-129-5p on NAFLD may function by regulating lipid accumulation and insulin sensitivity.

Key words

non-alcoholic fatty liver disease - insulin resistance - microRNA-129-5p - HOMA-IR


Publikationsverlauf

Eingereicht: 29. März 2021

Angenommen nach Revision: 11. August 2021

Artikel online veröffentlicht:
15. September 2021

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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