Clinical Value and Role of miR-129-5p in Non-Alcoholic Fatty Liver
Disease

Yuan Wang; Yujia Feng; Han Zhang; Qinghui Niu; Kun Liang; Cheng Bian; Hong Li

doi:10.1055/a-1587-9211

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2021; 53(10): 692-698
DOI: 10.1055/a-1587-9211

Endocrine Research

Clinical Value and Role of miR-129-5p in Non-Alcoholic Fatty Liver Disease

Yuan Wang‡

¹Sub-Health Clinic of Physical Examination Center, Beijing Fengtai Hospital of Integrated Traditional Chinese and Western Medicine, Beijing, China

,

Yujia Feng‡

²Department of Gastroenterology, People's Hospital of Rizhao, Rizhao, China

,

Han Zhang

³Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, China

,

Qinghui Niu

³Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, China

,

Kun Liang

⁴Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, China

,

Cheng Bian

⁴Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, China

,

Hong Li‡

⁵Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital,Tongji University School of Medicine, Shanghai, China

› Institutsangaben

Abstract

This study was to evaluate the expression of miR-129–5p in non-alcoholic fatty liver (NAFLD) patients and its clinical value and explore its regulatory effect on insulin resistance (IR). A total of 117 NAFLD patients and 110 healthy controls were included. The levels of miR-129-5p were detected by qRT-PCR. To assess the diagnostic value of miR-129-5p for NAFLD, the receiver operating characteristic curve (ROC) was established. C57Bl/6 mice were supplied with high-fat diet to establish NAFLD model. Intraperitoneal insulin tolerance test (IPITT) was carried out to evaluate the effect of miR-129-5p on IR in NAFLD animal model. miR-129-5p was highly expressed in the serum of NAFLD patients, and patients with HOMA-IR ≥2.5 had higher level of miR-129-5p than those with HOMA-IR <2.5. miR-129-5p had the ability to differentiate NAFLD patients from healthy individuals and might be associated with the development of IR. Serum miR-129-5p was positively correlated with the levels of HOMA-IR, BMI, total cholesterol (TC), and triglyceride (TG) in NAFLD patients. Downregulation of miR-129-5p regulates lipid metabolism and insulin sensitivity in NAFLD mice model. MiR-129-5p was upregulated in NAFLD patients and might be a potential diagnostic biomarker. The regulatory effect of miR-129-5p on NAFLD may function by regulating lipid accumulation and insulin sensitivity.

Key words

non-alcoholic fatty liver disease - insulin resistance - microRNA-129-5p - HOMA-IR

Volltext

Referenzen

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