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Planta Med 2009; 75(1): 32-36
DOI: 10.1055/s-0028-1088360
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Antigenotoxic Effects of the Disulfide Compound Persicasulfide A (PSA) on Rat Lymphocytes Exposed to Oxidative Stress

Saeid Noroozi1 , 2 , Fatemeh Mosaffa1 , 2 , Fatemeh Soltani1 , 2 , Mehrdad Iranshahi1 , 2 , Gholamreza Karimi3 , 4 , Mohammad Malekaneh5 , Fatemeh Haghighi6 , Javad Behravan1 , 2
  • 1Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • 2Department of Pharmacognosy and Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • 3Department of Pharmacodinamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • 4Toxcicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • 5Department of Clinical Biochemistry, Birjand University of Medical Sciences, Birjand, Iran
  • 6Department of Pathology, Birjand University of Medical Sciences, Birjand, Iran
Further Information

Publication History

Received: April 30, 2008 Revised: September 4, 2008

Accepted: September 8, 2008

Publication Date:
21 November 2008 (online)

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Abstract

The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by hydrogen peroxide (H2O2) was evaluated using single cell gel electrophoresis (SCGE). PSA was extracted from F. persica, characterized by NMR and its antioxidant/antigenotoxic effects were investigated. The antigenotoxic effect of solutions containing either PSA (1, 10, 50, 100, 200, 300, 400 and 500 μM) or ascorbic acid (250, 500, 750 and 1000 μM) alone, or in the presence of H2O2 (25, 50, 100 and 200 μM) were tested on lymphocytes derived from the blood of healthy male Wistar rats (250 – 300 g) by using the comet assay. The degree of damage to DNA after exposure to different solutions was calculated based on the amount of DNA present in the tail compared to the total amounts of lymphocyte DNA. PSA did not show genotoxicity and caused a 50 % reduction in DNA damage induced by H2O (EC50 : 476.47 ± 67.46 μM). Compared to the EC50 for ascorbic acid (1399.23 ± 205.21 μM), it was deduced that PSA was more effective than ascorbic acid in the prevention of oxidative damage to DNA.

Key words

Ferula persica - Apiaceae - antigenotoxicity - comet assay - persicasulfide A

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J. Behravan

Department of Pharmacognosy and Biotechnology

School of Pharmacy

Mashhad University of Medical Sciences

P. O. Box 91775

1365 Mashhad

Iran

Phone: +98-511-882 3255

Fax: +98-511-882 3251

Email: behravanj@mums.ac.ir