Abstract
The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by hydrogen peroxide (H2O2) was evaluated using single cell gel electrophoresis (SCGE). PSA was extracted from
F. persica, characterized by NMR and its antioxidant/antigenotoxic effects were investigated.
The antigenotoxic effect of solutions containing either PSA (1, 10, 50, 100, 200,
300, 400 and 500 μM) or ascorbic acid (250, 500, 750 and 1000 μM) alone, or in the
presence of H2O2 (25, 50, 100 and 200 μM) were tested on lymphocytes derived from the blood of healthy
male Wistar rats (250 – 300 g) by using the comet assay. The degree of damage to DNA
after exposure to different solutions was calculated based on the amount of DNA present
in the tail compared to the total amounts of lymphocyte DNA. PSA did not show genotoxicity
and caused a 50 % reduction in DNA damage induced by H2O (EC50 : 476.47 ± 67.46 μM). Compared to the EC50 for ascorbic acid (1399.23 ± 205.21 μM), it was deduced that PSA was more effective
than ascorbic acid in the prevention of oxidative damage to DNA.
Key words
Ferula persica
- Apiaceae - antigenotoxicity - comet assay - persicasulfide A
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J. Behravan
Department of Pharmacognosy and Biotechnology
School of Pharmacy
Mashhad University of Medical Sciences
P. O. Box 91775
1365 Mashhad
Iran
Phone: +98-511-882 3255
Fax: +98-511-882 3251
Email: behravanj@mums.ac.ir
