Protective Effects of Echinacoside, One of the Phenylethanoid Glycosides, on H2O2-Induced Cytotoxicity in PC12 Cells

Rong Kuang; Yiguo Sun; Wei Yuan; Li Lei; Xiaoxiang Zheng

doi:10.1055/s-0029-1185806

Planta Medica, Table of Contents

Planta Med 2009; 75(14): 1499-1504
DOI: 10.1055/s-0029-1185806

Pharmacology

Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Protective Effects of Echinacoside, One of the Phenylethanoid Glycosides, on H₂O₂-Induced Cytotoxicity in PC12 Cells

Rong Kuang¹ ^, ² , Yiguo Sun¹ , Wei Yuan¹ , Li Lei³ , Xiaoxiang Zheng¹

¹Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering, Ministry of Education, Zhejiang University (Yuquan Campus), Hang Zhou, People's Republic of China
²Zhejiang Food and Drug Institute for Control, Hang Zhou, People's Republic of China
³Sinphar Tianli Pharmaceutical Co., Ltd., Yuhang Economic Development Zone, Hang Zhou, People's Republic of China

Abstract

We have investigated the protective effects of echinacoside (ECH), one of the phenylethanoid glycosides, on H₂O₂-induced cytotoxicity in the rat pheochromocytoma cell line (PC12 cells). Our data show that application of ECH to H₂O₂-injured PC12 cells (HIPCs) increased cell viability and decreased the apoptotic ratio. Flow cytometry (FCM) and laser scanning confocal microscopy (LSCM) analysis suggested that ECH exerted its inhibitory effects on the formation of reactive oxygen species (ROS) and the accumulation of intracellular free Ca²⁺ ([Ca²⁺]i). In addition, ECH elevated the mitochondrial membrane potential (MMP) in HIPCs. Furthermore, Western blot analysis revealed that ECH prevented an H₂O₂-induced increase of the Bax/Bcl-2 ratio by down-regulating Bax protein expression and upregulating Bcl-2 protein expression. In summary, ECH showed significant neuroprotective effects on HIPCs through the mitochondrial apoptotic pathway, and could be a potential candidate for intervention in neurodegenerative diseases such as Alzheimer's and Parkinson's disease.

Key words

echinacoside (ECH) - apoptosis - ROS - MMP - [Ca²⁺]i - Bax/Bcl‐2

Full Text

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Professor Xiaoxiang Zheng

Department of Biomedical Engineering
Key Laboratory of Biomedical Engineering
Ministry of Education
Zhejiang University

Zheda Road 38

310027 Hang Zhou

People's Republic of China

Phone: + 86 5 71 87 95 38 60

Fax: + 86 5 71 87 95 16 76

Email: zxx@mail.bme.zju.edu.cn