Three Major Metabolites of Mulberroside A in Rat Intestinal Contents and Feces

Abstract

Mulberroside A, a major stilbene constituent of Morus alba L. (Moraceae), displays significant antitussive and antiasthmatic effects in animals. As part of our ongoing research on its biotransformation in rats, mulberroside A was orally administered to rats, and the metabolites in the gastrointestinal contents and feces were investigated. Three major metabolites were isolated from the feces of rats and identified as oxyresveratrol-2-O-β-D-glucopyranoside (1), oxyresveratrol-3′-O-β-D-glucopyranoside (2), and oxyresveratrol (3) on the basis of chemical and spectroscopic evidence. The three metabolites were also detected in the small intestinal contents of rats following oral administration of mulberroside A. These findings suggest that mulberroside A is metabolized prior to absorption into the body.

Key words

Moraceae - Morus alba - mulberroside A - metabolites - gastrointestinal contents - feces

References

• 1 Tian D H. Shi Yong Zhong Yao Ci Dian. Cortex Mori. Beijing; People's Health Presp 2005: 1666-1669
• 2 Piao S J, Qu G X, Qiu F. Chemical constituents of the water extract from Cortex Mori.  Zhongguo Yaowu Huaxue Zazhi. 2006;  16 40-45
• 3 Kan Q M, Kang N, Tian H T, Li C L, Qiu F, Liu Y L. Antitussive and antiasthmatic effects of mulberroside A.  Shenyang Yaoke Daxue Xuebao. 2006;  23 388-391
• 4 Qiu F, Komatsu K I, Saito K I, Kawasaki K, Yao X S, KanoY. Pharmacological properties of traditional medicines. XXII. Pharmacokinetic study of mulberroside A and its metabolites in rat.  Biol Pharm Bull. 1996;  19 1463-1467
• 5 Qiu F, Komatsu K, Saito K, Kawasaki K, Yao X S, Kano Y. Pharmacological properties of traditional medicines. XXI. Analysis of plasma, urine and bile of rats after oral administration of water extract of Mori Cortex.  Nat Med. 1996;  50 103-108
• 6 Kanchanapoom T, Suga K, Kasai R, Yamasaki K, Kamel M S, Mohamed M H. Stilbene and 2-arylbenzofuran glucosides from the rhizomes of Schoenocaulon officinale.  Chem Pharm Bull. 2002;  50 863-865
• 7 Qiu F, Kenichi K, Kanako K, Kenichi S, Yao X S, Yonshihiro K. A novel stilbene glucoside, oxyresveratrol 3′-β-O-glucopyranoside, from the root bark of Morus alba.  Planta Med. 1996;  62 559-561
• 8 Qiu F, Kano Y, Yao X S. Elucidation of the bioactive constituents in traditional Chinese medicine Mori Cortex. Studies in plant science 6, advances in plant glycosides. Proceedings of the International Symposium on Plant Glycosides, Kunming, China. 1997

1 These authors contributed equally to this work.