African Descents Are More Sensitive Than European Descents to the Antitumor Compounds α-Hederin and Kalopanaxsaponin I

Geva Feller; Aleksandra Kugel; Dana Moonshine; Vered Chalifa-Caspi; Martin Scholz; Dirk Prüfer; Tatiana Rabinski; Kai J. Müller; Rivka Ofir

doi:10.1055/s-0030-1250061

Planta Medica, Table of Contents

Planta Med 2010; 76(16): 1847-1851
DOI: 10.1055/s-0030-1250061

Pharmacology

Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

African Descents Are More Sensitive Than European Descents to the Antitumor Compounds α-Hederin and Kalopanaxsaponin I

Geva Feller¹ , Aleksandra Kugel¹ , Dana Moonshine¹ , Vered Chalifa-Caspi¹ , Martin Scholz² , Dirk Prüfer² ^, ³ , Tatiana Rabinski⁴ , Kai J. Müller³ , Rivka Ofir⁴

¹Ben Gurion University of the Negev, Bioinformatic Core Facility, National Institute of Biotechnology in the Negev, Beer Sheva, Israel
²Institute for Biochemistry and Biotechnology of Plants (IBBP), Westphalian Wilhelms-University Münster, Münster, Germany
³Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
⁴Dead Sea & Arava Science Center, 86910, Israel, and Department of Immunology and Microbiology, Health Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel

Abstract

α-Hederin, a natural triterpene saponin and its derivative kalopanaxsaponin I (ksI) exhibit cytotoxicity against various cancer cell lines and in vivo tumors. We studied the genetic variants contributing to the activity of these two anticancer compounds. Cell lines derived from 30 trios of European descent (Centre d'Etude du Polymorphisme Human, CEPH; CEU) and 30 trios of African descent (Yoruban, YRI) were used. Cytotoxicity was determined as inhibition of cell growth at increasing concentrations of α-hederin or ksI for 24 h. In comparison to the European, the Yoruban populations revealed a higher sensitivity to α-hederin and to ksI that can be attributed to several unique SNPs. These SNPs are located near 111 and 130 genes in the European and the Yoruban populations, respectively, raising the possibility that some of these genes contribute to the differential sensitivity to these compounds.

Key words

α‐hederin - kalopanaxsaponin I - Nigella sativa - Ranunculaceae - HapMap - lymphoblastoid cell lines - whole genome association

Full Text

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Dr. Rivka Ofir

Dead Sea & Arava Science Center, 86910 Israel and
Department of Immunology and Microbiology, Health Sciences
Ben Gurion University of the Negev

P. O. Box 653

84105 Beer Sheva

Israel

Phone: + 97 25 23 44 89 82

Fax: + 9 72 86 58 20 68

Email: rivir@bgu.ac.il