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Synthesis 2010(21): 3710-3714  
DOI: 10.1055/s-0030-1258243
PAPER
© Georg Thieme Verlag Stuttgart ˙ New York

Synthesis of Readily Accessible Triazole-Linked Dimer Deoxynucleoside Phosphoramidite for Solid-Phase Oligonucleotide Synthesis

Srivari Chandrasekhar*a, Pabbaraja Sriharia, Chidalla Nagesha, Nayani Kiranmaia, Narayana Nageshb, M. Mohammed Idris*b
a Organic Chemistry Division - I, Indian Institute of Chemical Technology, Hyderabad 500 007, India
Fax: +91(40)27160512; e-Mail: srivaric@iict.res.in;
b Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
e-Mail: idris@ccmb.res.in.;
Further Information

Publication History

Received 25 June 2010
Publication Date:
07 September 2010 (online)

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Abstract

A concise preparation of triazole-linked deoxynucleoside phosphoramidite for its direct use in solid-phase oligonucleo­tide synthesis is reported. This dimer has successfully been utilized in solid-phase synthesis to make the 10- and 12-mer oligomers.

Key words

nucleotides - triazole - click - oligomer - solid-phase synthesis

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19

Initially, the reaction was tried with n-BuLi and the yield was found to be poor as the reaction ended with an intractable mixture of products.