Synthesis of Readily Accessible
Triazole-Linked Dimer Deoxynucleoside Phosphoramidite
for Solid-Phase Oligonucleotide Synthesis

Srivari Chandrasekhar; Pabbaraja Srihari; Chidalla Nagesh; Nayani Kiranmai; Narayana Nagesh; M. Mohammed Idris

doi:10.1055/s-0030-1258243

PAPER

Synthesis of Readily Accessible Triazole-Linked Dimer Deoxynucleoside Phosphoramidite for Solid-Phase Oligonucleotide Synthesis

Srivari Chandrasekhar*^a, Pabbaraja Srihari^a, Chidalla Nagesh^a, Nayani Kiranmai^a, Narayana Nagesh^b, M. Mohammed Idris*^b
^a Organic Chemistry Division - I, Indian Institute of Chemical Technology, Hyderabad 500 007, India
Fax: +91(40)27160512; e-Mail: srivaric@iict.res.in;
^b Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
e-Mail: idris@ccmb.res.in.;

Abstract

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Abstract

A concise preparation of triazole-linked deoxynucleoside phosphoramidite for its direct use in solid-phase oligonucleotide synthesis is reported. This dimer has successfully been utilized in solid-phase synthesis to make the 10- and 12-mer oligomers.

Key words

nucleotides - triazole - click - oligomer - solid-phase synthesis

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References

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Initially, the reaction was tried with n-BuLi and the yield was found to be poor as the reaction ended with an intractable mixture of products.