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Planta Med 2011; 77(15): 1742-1748
DOI: 10.1055/s-0030-1271037
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Quantitative Determination of Triperpene Saponins and Alkenated-Phenolics from Labisia pumila Using an LC-UV/ELSD Method and Confirmation by LC-ESI-TOF

Bharathi Avula1 , Yan-Hong Wang1 , Zulfiqar Ali1 , Troy J. Smillie1 , Ikhlas A. Khan1 , 2
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA
  • 2Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS, USA
Further Information

Publication History

received Dec. 22, 2010 revised March 30, 2011

accepted April 1, 2011

Publication Date:
17 May 2011 (online)

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Abstract

This study describes the first analytical method for the determination of four triterpene saponins (ardisicrenoside B, ardisiacrispin A, 3-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranosyl-(1 → 4)-α-L-arabinopynanosyl cyclamiretin A and ardisimamilloside H) and three alkenated-phenolics (irisresorcinol, belamcandol B, and demethylbelamcandaquinone B) from the leaves, leaves/stems, and roots of Labisia pumila using an HPLC-UV-ELSD method. The separation was achieved using a reversed-phase (C-18) column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. The major triterpenoid (ardisiacrispin A) and irisresorcinol compounds were detected at a concentration as low as 10.0 and 0.2 µg/mL, respectively. Analysis of various samples showed considerable variation of 0.11–2.46 % for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with electrospray ionization (ESI) is described for the identification of compounds in plant samples. This method involved the use of the [M + Na]+ and [M + NH4]+ ions for compounds 1–4 in the positive ion mode with extractive ion chromatogram (EIC).

Key words

Labisia pumila - Myrsinaceae - LC-UV-ELSD - LC-MS

Supporting Information

References

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Prof. Dr. Ikhlas A. Khan

National Center for Natural Products Research
Research Institute of Pharmaceutical Sciences
The University of Mississippi

P. O. Box 1848

University, MS 38677

USA

Phone: +1 66 29 15 78 21

Fax: +1 66 29 15 79 89

Email: ikhan@olemiss.edu