Abstract
This study describes the first analytical method for the determination of four triterpene saponins (ardisicrenoside B, ardisiacrispin A, 3-O -α -L -rhamnopyranosyl-(1 → 2)-β -D -glucopyranosyl-(1 → 4)-α -L -arabinopynanosyl cyclamiretin A and ardisimamilloside H) and three alkenated-phenolics (irisresorcinol, belamcandol B, and demethylbelamcandaquinone B) from the leaves, leaves/stems, and roots of Labisia pumila using an HPLC-UV-ELSD method. The separation was achieved using a reversed-phase (C-18) column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. The major triterpenoid (ardisiacrispin A) and irisresorcinol compounds were detected at a concentration as low as 10.0 and 0.2 µg/mL, respectively. Analysis of various samples showed considerable variation of 0.11–2.46 % for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with electrospray ionization (ESI) is described for the identification of compounds in plant samples. This method involved the use of the [M + Na]+ and [M + NH4 ]+ ions for compounds 1 –4 in the positive ion mode with extractive ion chromatogram (EIC).
Key words
Labisia pumila
- Myrsinaceae - LC-UV-ELSD - LC-MS
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ikhan@olemiss.edu
