Abstract
Two new tirucallane triterpenoids, 24,25-epoxy-3β-hydroxy-20-oxo-7-tirucallene (1) and 22,23;24,25-diepoxy-3β-hydroxy-7-tirucallene (2), and a new tetranortriterpenoid, 4α-hydroperoxy-6-O-acetylnimbandiol (3), along with eight known compounds, were isolated from the branches and leaves of Azadirachta indica. Their structures were elucidated through spectroscopic and chemical methods. The cytotoxic assay showed that the abundant constituent nimbolide (8) had obvious cytotoxic activities against HL-60, SMMC7721, A549, MCF-7, and SW-480 cell lines, with IC50 values of 0.8 ± 0.1, 2.2 ± 0.2, 1.9 ± 1.3, 4.5 ± 1.1, and 2.3 ± 0.1 µM, respectively.
Key words
Meliaceae -
Azadirachta indica
- tirucallane triterpenoid - tetranortriterpenoid - cytotoxicity
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MingHua Qiu
State Key Laboratory of Phytochemistry and Plant Resources in West China
Kunming Institute of Botany, Chinese Academy of Sciences
132, LanHei Road
Kunming 650204, Yunnan
P. R. China
Phone: +86 871 5 22 33 27
Fax: +86 871 5 22 32 55
Email: mhchiu@mail.kib.ac.cn
