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Synlett 2013; 24(1): 20-23
DOI: 10.1055/s-0032-1317728
DOI: 10.1055/s-0032-1317728
letter
Facile Synthetic Method of Alkanethiol Spacer for Biointerface
Further Information
Publication History
Received: 11 September 2012
Accepted after revision: 12 November 2012
Publication Date:
10 December 2012 (online)
Abstract
An alkanethiol spacer which is suitable for biointerface was synthesized on 2-chlorotritylchloride (CTC) resin using solid-phase synthesis method. The triethylene glycol (TEG) unit containing building block was immobilized on CTC resin followed by a coupling alkanethiol building block. After dithiothreitol (DTT) treatment and cleavage of the resulting alkanethiol spacer from the resin, the spacer was obtained in a pure form without any purification steps. The TEG-alkanethiol spacer was applied for the DNA hybridization assay on a gold surface, and proved to work well when compared to the commercial spacer.
Key words
alkanethiol - solid-phase synthesis - self-assembled monolayer - biointerface - nonspecific adsorptionSupporting Information
- for this article is available online at http://www.thieme-connect.com/ejournals/toc/synlett.
- Supporting Information
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