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Thromb Haemost 2002; 87(03): 442-449
DOI: 10.1055/s-0037-1613024
Review Article
Schattauer GmbH

Procoagulant Activity of T Lymphoblastoid Cells due to Exposure of Negatively Charged Phospholipid

T.W. Barrowcliffe
1   National Institute for Biological Standards and Control (NIBSC), Potters Bar, UK
,
P. Fábregas
2   Institutde Recerca Oncològica (IRO), Barcelona, Spain
,
M. Jardi
2   Institutde Recerca Oncològica (IRO), Barcelona, Spain
,
J. Cancelas
2   Institutde Recerca Oncològica (IRO), Barcelona, Spain
,
M. Rabaneda
2   Institutde Recerca Oncològica (IRO), Barcelona, Spain
,
J. Felez
2   Institutde Recerca Oncològica (IRO), Barcelona, Spain
› Author Affiliations
Further Information

Publication History

Received 29 May 2001

Accepted after revision 16 December 2001

Publication Date:
14 December 2017 (online)

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Summary

We have characterised the Procoagulant activity (PCA) of six well-established cell lines by assays of tissue factor (TF), thrombin and FXa generation, flow cytometry using Annexin V, and by binding studies with Factors VIII and IXa.

The monocytic (THP-1 & U937) and promyelocytic (NB4) cells expressed high concentrations of TF antigen and activity, whereas TF in the lymphocytic cells (Molt 4, Jurkat & Nalm 6) was very low or absent. However the T-lymphoblastoid cells (Molt 4 & Jurkat) promoted the generation of large amounts of thrombin despite their low TF content, and these cells were also the most active in supporting Factor Xa generation. Molt 4 cells bound Factors VIII and IXa with high capacity and their activity was inhibited by Annexin V. These results indicate that the PCA of T-lymphoblastoid lines is due to expression of negatively charged phospholipids. Flow cytometry studies showed Annexin V binding to the major population of nonapoptotic Molt 4 cells and the PCA of Molt 4 was not increased when apoptosis was induced by staurosporine, indicating that PCA is independent of apoptotic status.

Keywords

T cells - coagulation - phospholipid
 

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