Early Embryonic Expression of Murine Coagulation System Components

Kelly Ong; Wendy Horsfall; Edward M. Conway; Andre C. Schuh

doi:10.1055/s-0037-1614166

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2000; 84(06): 1023-1030
DOI: 10.1055/s-0037-1614166

Review Article

Schattauer GmbH

Early Embryonic Expression of Murine Coagulation System Components

Kelly Ong

¹From the Institute of Medical Sciences, Toronto, Ontario, Canada

,

Wendy Horsfall

¹From the Institute of Medical Sciences, Toronto, Ontario, Canada

²The Departments of Medicine, Toronto, Ontario, Canada

,

Edward M. Conway

¹From the Institute of Medical Sciences, Toronto, Ontario, Canada

²The Departments of Medicine, Toronto, Ontario, Canada

³Medical Biophysics, Toronto, Ontario, Canada

⁴University of Toronto, and the Division of Hematology/Oncology, The Toronto Hospital, Toronto, Ontario, Canada

⁵Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, University of Leuven, Leuven, Belgium

,

Andre C. Schuh

¹From the Institute of Medical Sciences, Toronto, Ontario, Canada

²The Departments of Medicine, Toronto, Ontario, Canada

³Medical Biophysics, Toronto, Ontario, Canada

⁴University of Toronto, and the Division of Hematology/Oncology, The Toronto Hospital, Toronto, Ontario, Canada

› Author Affiliations

Abstract

Summary

The development of the embryonic coagulation system, and its contribution to the maintenance of vascular integrity during the formation of embryonic blood vessels, remain poorly understood. We have characterized the temporal expression patterns of 27 hemostasis-related genes during murine development. We show that, although most coagulation and fibrinolysis-related factors are expressed coordinately b 7.5 dpc, several, including FIX, FXII and PAI2, are not detectable until later developmental timepoints. The expression of hemostasis-specific genes prior to the formation of a functional circulatory system supports the view that some coagulation factors have additional non-hemostatic functions during development. In addition, the discordant expression of some factors suggests that the embryonic hemostatic system may be distinct from that of the adult. These analyses will help to elucidate the regulation of hemostasis during embryonic/vascular development, and will provide a framework to facilitate the interpretation of gene inactivation studies.

Keywords

Embryo - hemostasis - vascular development - knock-out

Full Text

References

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