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Thromb Haemost 1998; 79(01): 94-98
DOI: 10.1055/s-0037-1614227
DOI: 10.1055/s-0037-1614227
Review Article
Rapid Detection of D-dimer Using a Fiber Optic Biosensor
Weitere Informationen
Publikationsverlauf
Received
08. April 1997
Accepted after resubmission
04. September 1997
Publikationsdatum:
08. Dezember 2017 (online)
Summary
We describe a rapid and sensitive method for detection and quantification of D-dimer and other crosslinked fibrin degradation products (XL-FDPs), which are present in elevated concentrations in patients with sepsis and thrombotic disorders. The method utilizes a sandwich fluoroimmunoassay immobilized in the sensing region of an evanescent wave biosensor. Physiological concentrations of D-dimer and high molecular weight XL-FDP could be determined in buffer and plasma samples on calibrated fibers in 11 min. Samples from septic patients were assayed using ELISA and the fiber optic method; concentrations determined by fiber optic assay were strongly correlated with those determined by ELISA (r = 0.918); intra- and inter-assay errors were comparable to those from ELISAs. Given its accuracy and rapid response time, this fiber optic biosensor shows great potential for development as a diagnostic tool.
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