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Thromb Haemost 1999; 82(06): 1757-1763
DOI: 10.1055/s-0037-1614910
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Endothelialization of Crosslinked Albumin-heparin Gels

Gert W. Bos
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
,
Nicole M. Scharenborg
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
,
André A. Poot
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
,
Gerard H. M. Engbers
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
,
Tom Beugeling
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
,
Willem G. van Aken
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
,
Jan Feijen
1   From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 22 October 1998

Accepted after resubmission 22 June 1999

Publication Date:
10 December 2017 (online)

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Summary

Crosslinked gels of albumin as well as heparinized albumin gels, potential sealants of prosthetic vascular grafts, were studied with regard to in vitro stability, binding of basic fibroblast growth factor (bFGF) and cellular interactions. A small percentage of the heparin present in these gels, was released during storage in SDS solution. During storage in cell culture medium at 37° C, heparin release was 21-25 percent. Release of albumin did not occur.

Human umbilical vein endothelial cells (HUVECs) rapidly adhered and subsequently spread on (heparinized) albumin gels, but proliferation was only observed if heparin was present in the gel.

Binding of 125I-bFGF to heparinized albumin gel was 35 percent higher than to non-heparinized albumin gel. Growth of HUVECs occurred only on heparinized albumin gel loaded with bFGF and not on bFGF-loaded albumin gel.

The number of platelets deposited under stationary conditions onto heparinized albumin gel was about twice the number found on nonheparinized albumin gel. Seeding of HUVECs on heparinized albumin gel, significantly reduced the number of platelets adhering to this surface. Moreover, no spreading of platelets was observed on substrates seeded with HUVECs.

It can be concluded that crosslinked gels of albumin to which heparin is immobilized, are candidate sealants for prosthetic vascular grafts and suitable substrates for endothelial cell seeding.

Keywords

Endothelialization - vascular prosthesis - bFGF - platelet deposition
 

  • References

  • 1 Yeager A, Callow AD. New graft materials and current approaches to an acceptable small diameter vascular graft. Trans Am Soc Artif Intern Organs 1988; 34: 88-94.
    CrossrefPubMedSearch in Google Scholar
  • 2 Uchida N, Kambic H, Emoto H, Chen J-F, Hsu S, Murabachi S, Harasaki H, Nose Y. Compliance effects on small diameter polyurethane graft patency. J Biomed Mater Res 1993; 27: 1269-79.
    CrossrefPubMedSearch in Google Scholar
  • 3 Kodama M. Small-diameter cardiovascular graft. Proc Jpn Congr Mater Res 1990; 33: 153-5.
    PubMedSearch in Google Scholar
  • 4 Schmidt SP, Sharp WV, Evancho MM, Meerbaum SO. Endothelial cell seeding of prosthetic vascular grafts – current status. In: High performance biomaterials. Szycher M. ed. Lancaster, Pa: Technomic; 1991: 483-96.
    Search in Google Scholar
  • 5 Szilagyi DE, Elliot JP, Smith RF, Reddy DJ, McPharlin M. A thirty-year survey of the reconstructive surgical treatment of aortoiliac occlusive disease. J Vasc Surg 1986; 3: 421-36.
    CrossrefPubMedSearch in Google Scholar
  • 6 Wu MHD, Shi Q, Wechezak AR, Clowes AW, Gordon IL, Sauvage LR. Definitive proof of endothelialization of a dacron arterial prosthesis in a human being. J Vasc Surg 1995; 21: 862-7.
    CrossrefPubMedSearch in Google Scholar
  • 7 Antiplatelet Trialist’s Collaboration. Collaborative overview of randomised trials of antiplatelet therapy – II: Maintenance of vascular graft or arterial patency by antiplatelet therapy. Brit Med J 1994; 308: 159-68.
    CrossrefPubMedSearch in Google Scholar
  • 8 Davies MG, Hagen PO. The vascular endothelium – a new horizon. Ann Surg 1993; 218: 593-609.
    CrossrefPubMedSearch in Google Scholar
  • 9 Wu KK, Frasier-Scott K, Hadzakis H. Endothelial cell function in hemostasis and thrombosis. Adv Exp Med Biol 1988; 242: 127-33.
    PubMedSearch in Google Scholar
  • 10 James NL, Schindhelm K, Slowiaczek P, Milthorpe BK, Dudman NPB, Johnson G, Steele JG. Endothelial cell seeding of small diameter vascular grafts. Art Organs 1990; 14: 355-60.
    CrossrefPubMedSearch in Google Scholar
  • 11 Örtenwall P, Wadenvik H, Kutti J, Risberg B. Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans. J Vasc Surg 1990; 11: 403-10.
    CrossrefPubMedSearch in Google Scholar
  • 12 Zilla P, Deutsch M, Meinhart J, Puschmann R, Eberl T, Minar E, Dudczak R, Lugmaier H, Schmid P, Noszian I, Fischlein T. Clinical in vitro endothelialization of femoropopliteal bypass grafts: an actuarial follow-up over three years. J Vasc Surg 1994; 19: 540-8.
    CrossrefPubMedSearch in Google Scholar
  • 13 Meinhart J, Deutsch M, Zilla P. Eight years of clinical endothelial cell transplantation – Closing the gap between prosthetic grafts and vein grafts. Am Soc Artif Intern Organs 1997; 43: M515-21.
    PubMedSearch in Google Scholar
  • 14 Bos GW, Scharenborg NM, Poot AA, Engbers GHM, Terlingen JGA, Beugeling T, Van Aken WG, Feijen J. Adherence and proliferation of endothelial cells on surface-immobilized albumin-heparin conjugate. Tissue Engineering 1998; 4: 267-79.
    CrossrefPubMedSearch in Google Scholar
  • 15 Bos GW, Scharenborg NM, Poot AA, Engbers GHM, Beugeling T, Van Aken WG, Feijen J. Proliferation of endothelial cells on surface-immobilized albumin-heparin conjugate loaded with basic fibroblast growth factor. J Biomed Mater Res 1999; 44: 330-40.
    CrossrefPubMedSearch in Google Scholar
  • 16 Prestrelski SJ, Fox GM, Arakawa T. Binding of heparin to basic fibroblast growth factor induces a conformational change. Arch Biochem Biophys 1992; 293: 314-9.
    CrossrefPubMedSearch in Google Scholar
  • 17 Feige JJ, Bradley JD, Fryburg K, Farris J, Cousens LC, Barr PJ, Baird A. Differential effects of heparin, fibronectin, and laminin on the phosphorylation of basic fibroblast growth factor by protein kinase C and the catalytic subunit of protein kinase A. J Cell Biol 1989; 109: 3105-14.
    CrossrefPubMedSearch in Google Scholar
  • 18 Sommer A, Rifkin DB. Interaction of heparin with human basic fibroblast growth factor : protection of the angiogenic protein from proteolytic degradation by a glycosaminoglycan. J Cell Phys 1989; 138: 215-20.
    CrossrefPubMedSearch in Google Scholar
  • 19 Yayon A, Klagsbrun M, Esko JD, Leder P, Ornitz DM. Cell surface, heparin-like molecules are required for binding of basic fibroblast growth factor to its high affinity receptor. Cell 1991; 64: 841-8.
    CrossrefPubMedSearch in Google Scholar
  • 20 Bos GW, Poot AA, Engbers GHM, Beugeling T, Van Aken WG, Feijen J. Small-diameter vascular grafts-current status. Arch Physiol Biochem 1998; 106: 100-15.
    CrossrefPubMedSearch in Google Scholar
  • 21 Ben Slimane S, Guidoin R, Merhi Y, King MW, Domurado D, Sigot-Luizard MF. In vivo evaluation of polyester arterial grafts coated with albumin: the role and importance of crosslinking agents. Eur Surg Res 1988; 20: 66-74.
    CrossrefPubMedSearch in Google Scholar
  • 22 Guidoin R, Marceau D, Rao TJ, King M, Merhi Y, Roy PE, Martin L, Duval M. In vitro and in vivo characterization of an impervious polyester arterial prosthesis: the Gelseal Triaxial graft. Biomaterials 1987; 8: 433-41.
    CrossrefPubMedSearch in Google Scholar
  • 23 Hirt SW. Comparative in vivo study on the healing qualities of four different presealed vascular prostheses. Biomaterials 1993; 10: 156-65.
    PubMedSearch in Google Scholar
  • 24 Merhi Y, Roy R, Guidoin R, Hebert J, Mourad W, Benslimane S. Cellular reactions to polyester arterial prostheses impregnated with crosslinked albumin: in vivo studies in mice. Biomaterials 1989; 10: 56-8.
    CrossrefPubMedSearch in Google Scholar
  • 25 Hatton MWC, Berry LR, Machovich R, Regoeczi E. Titration of commercial heparins by reaction with NaB3H4: chemical analysis and biological properties of the product. Anal Biochem 1983; 106: 417-26.
    PubMedSearch in Google Scholar
  • 26 Jaffe EA, Nachman RL, Becker CG, Miniick CR. Culture of human endothelial cells derived from umbilical cord veins Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-56.
    CrossrefPubMedSearch in Google Scholar
  • 27 Markwell MAK. A new solid-state reagent to iodinate proteins: conditions for the efficient labeling of antiserum. Anal Biochem 1982; 125: 427-32.
    CrossrefPubMedSearch in Google Scholar
  • 28 Bos GW, Scharenborg NM, Poot AA, Engbers GHM, Beugeling T, Van Aken WG, Feijen J. Blood compatibility of and in vitro platelet adhesion onto albumin-heparin conjugate monolayers on carbon dioxyde plasma treated polystyrene. J Biomed Mater Res. 1999 in press.
    PubMedSearch in Google Scholar
  • 29 Laemmel E, Penhoat J, Warocquier-Clérout R, Sigot-Luizard MF. Heparin immobilized on proteins usable for arterial prostheses coating: growth inhibition of smooth-muscle cells. J Biomed Mater Res 1998; 39: 446-52.
    CrossrefPubMedSearch in Google Scholar
  • 30 Albes JM, Klenzner T, Kotzerke J, Thiedemann KU, Schäfers HJ, Borst HG. Improvement of tracheal autograft revascularization by means of fibroblast growth factor. Ann Thorac Surg 1994; 57: 444-9.
    CrossrefPubMedSearch in Google Scholar
  • 31 Yamamura K, Sakurai T, Yano K, Nabeshima T, Yotsuyanagi T. Sustained release of basic fibroblast growth factor from the synthetic vascular prosthesis using hydroxypropylchitosan acetate. J Biomed Mater Res 1995; 29: 203-6.
    CrossrefPubMedSearch in Google Scholar
  • 32 Schneider PA, Hanson SR, Price TM, Harker LA. Preformed confluent endothelial cell monolayers prevent early platelet deposition on vascular prostheses in baboons. J Vasc Surg 1988; 8: 229-35.
    CrossrefPubMedSearch in Google Scholar
  • 33 Örtenwall P, Wadenvik H, Kutti J, Risberg B. Reduction in deposition of indium 111-labeled platelets after autologous endothelial cell seeding of Dacron aortic bifurcation grafts in humans: a prelimenary report. J Vasc Surg 1987; 6: 17-25.
    CrossrefPubMedSearch in Google Scholar
  • 34 Fasol R, Zilla P, Deutsch M, Grimm M, Fischlein T, Laufer G. Human endothelial cell seeding: evaluation of its effectiveness by platelet parameters after one year. J Vasc Surg 1989; 9: 432-6.
    CrossrefPubMedSearch in Google Scholar
  • 35 Dekker A, Poot AA, Beugeling T, Bantjes A, Van Aken WG. The effect of vascular cell seeding on platelet deposition in an in vitro capillary perfusion model. Thromb Haemost 1989; 61: 402-8.
    Thieme ConnectPubMedSearch in Google Scholar
  • 36 Klomp AJA. University of Twente, section of Polymer Chemistry and Biomaterials, personal communications..
    PubMed