Localization of ^99mTc-Diphosphonates in Newly Formed Bone Matrix as a Measure of Bone Lesion Detectability^[*]

The lesion-to-normal-bone ratios of DBA-MDP (dibutylamino-methylene-diphosphonate), DPD (dicarboxypropane-diphosphonate) and MDP (methylene-diphosphonate) each labeled with ^99mTc, were evaluated in experimental bone lesions. In 3-day old lesions this ratio was increased twofold for DBA-MDP in comparison with MDP and DPD which showed nearly equal ratios. Later on these differences became negligibly small. It is concluded that ^99mTc-DBA-MDP is fixed more strongly in the immature bone matrix and that this will lead to an improvement in the detectability of lesions containing larger amounts of immature bone matrix.

Zusammenfassung

Das Anreicherungsverhältnis zwischen experimentellen Knochenläsionen und normalem Knochen wurde für ^99mTc-markiertes DBA-MDP (Dibutylamino-Methylen-Diphosphonat), DPD (Dicarboxypropan-Diphosphonat) und MDP (Methylen-Diphosphonat) gemessen. Bei 3 Tage alten Läsionen war dieses Verhältnis bei ^99mTc-DBA-MDP um das Zweifache gegenüber MDP und DPD erhöht. Später wurden diese Unterschiede vernachlässigbar klein. Es wird die Schlußfolgerung gezogen, daß ^99mTc-DBA-MDP in der unreifen Knochenmatrix stärker fixiert wird, und daß deshalb die Nachweiswahrscheinlichkeit von Knochenläsionen, die größere Mengen an unreifer Knochenmatrix enthalten, verbessert wird.

• REFERENCES

• 1 Adler C-P. Knochenkrankheiten: Diagnostik makroskopischer, histologischer und radiologischer Strukturveränderungen des Skeletts. Stuttgart - New York: Thieme; 1983
• 2 Büll U, Kleinhans E, Zorn-Bopp E. et al. A comparison of bone imaging with ^99mTc DPD and ^99mTc MDP. J Nucl Med 1982; 23: 214-7.
• 3 Charkes N D. Mechanisms of skeletal tracer uptake. J Nucl Med 1979; 20: 794-5.
• 4 Delaloye B, Delaloye-Bischof A, Dudczak R. et al. Clinical comparison of ^99mTc-HMDP and ^99mTc-MDP A multicenter study. Eur J Nucl Med 1985; 11: 182-5.
• 5 Fogelman I, Citrin D L, McKillop J H. et al. A clinical comparison of ^99mTc HEDP and ^99mTc MDP in the detection of bone metastases. J Nucl Med 1979; 20: 98-101.
• 6 Guillemart A, Le Pape A, Galy G, Besnard J-C. Bone kinetics of calcium-45 and pyrophosphate labeled with technetium-96. An autoradiographic evaluation. J Nucl Med 1980; 21: 466-70.
• 7 Pauwels E K J, Blom J, Camps J A J, Hermans J, Rijke A M. A comparison between the diagnostic efficacy of ^99mTc-MDP and ^99mTc-HDP for the detection of bone metastases. Eur J Nucl Med 1983; 08: 118-22.
• 8 Rosenthall L, Stern J, Arzoumanian A. A clinical comparison of MDP and DMAD. Clin Nucl Med 1982; 07: 403-6.
• 9 Schwarz A, Kloss G. Technetium-99m-DPD - a new skeletal imaging agent. J Nucl Med 1981; 22: 77.
• 10 Schümichen C, Mundriziewski L, Tischler E, Hoffmann G. Relationship between blood flow and radiostrontium uptake in the healing bone fracture. Eur J Nucl Med 1979; 04: 413-7.
• 11 Schümichen C, Rempfle H, Wagner M, Hoffmann G. The short-term fixation of radiopharmaceuticals in bone. Eur J Nucl Med 1979; 04: 423-8.
• 12 Schümichen C, Koch K, Kraus A. et al. Binding of technetium-99m to plasma proteins. Influence on the distribution of Tc-99m phosphate agents. J Nucl Med 1980; 21: 1080-5.
• 13 Schümichen C, Schmidt H, Koutsomanis D. ^99mTc DPD (dicarboxypropane diphosphonate), mechanism of bone uptake and renal excretion. J Nucl Med 1982; 23: 77.
• 14 Schümichen C. Physiologische Grundlagen der Knochenszintigraphie; Meßtechnik und quantitative Auswertung. Der Nuklearmediziner 1984; 02: 73-88.
• 15 Smith M L, Martin W, McKillop J H. et al. Improved lesion detection with dimethylamino-diphosphonate. A report of two cases. Eur J Nucl Med 1984; 09: 519-20.
• 16 Subramanian G, McAfee J G, Blair R J, Thomas F D, Kallfelz E A. ^99mTc-methylene diphosphonate - a superior agent for skeletal imaging: Comparison with other ^99mTc-complexes. J Nucl Med 1975; 16: 744.
• 17 Unterspann S, Finck W. Chemical structure and pharmacokinetics of ^99mTc-labelled aminomethane diphosphonic acid derivatives. Eur J Nucl Med 1981; 06: 527.
• 18 Vaughan J M. The physiology of bone. Clarendon Press; Oxford: 1975
• 19 Vorne M, Vähätalo S, Lantto T. A clinical comparison of ^99mTc-DPD and two ^99mTc-MDP agents. Eur J Nucl Med 1983; 08: 395-7.
• 20 Wilson G M, Pinkerton T C. Determination of charge and size of technetium diphosphonate complexes by anion-exchange liquid chromatography. Anal Chem 1985; 57: 246.