Effect of Detergents on Platelet Electrophoresis and Aggregation, and the Possible Relation of Plasma Surface Activity to Thrombosis

Summary

A variety of anionic, cationic, and non-ionic detergent compounds has been shown to increase the sensitivity of the electrophoretic response of human platelets to adenosine diphosphate. The effect of such compounds on normal platelets is very similar to that of plasma from patients with arterial disease, and it is suggested that there may be an abnormality in the surface-active properties of the plasma from such patients. One detergent, cetyl pyridinium chloride, was found not to induce any change in platelet electrophoretic behaviour, but to protect platelets from other detergents. It was also shown to be a powerful inhibitor of platelet aggregation.

• References

• 1 Bolton C. H, Hampton J. R, and Mitchell J. R. A. 1967; The nature of the transferable factor that causes abnormal platelet behaviour in vascular disease. Lancet II: 1101.
• 2 Born G. V. R. 1962; Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (London) 194: 927.
• 3 Cunningham C. C, and Hager L. P. 1971; Crystalline pyruvic oxidase from Escherichia Coli: activation by phospholipids. Journal of Biological Chemistry 246: 1575.
• 4 Emmons P. R, and Mitchell J. R. A. 1965; Post-operative changes in platelet-clumping activity. Lancet 1: 11.
• 5 Hampton J. R, and Bolton C. H. 1969; Effect of phospholipids on platelet electrophoretic mobility. Journal of Atherosclerosis Research 9: 131.
• 6 Hampton J. R, Harrison M. J. G, Honour A. J, Mitchell J. R. A, and Prichard J. S. 1972 The assessment of anti-thrombotic agents: effects of dipyridamole analogues on platelet behaviour. Cardiovascular Research (in press).
• 7 Hampton J. R, and Mitchell J. R. A. 1966; a Effect of aggregating agents on platelet electrophoretic behaviour. British Medical Journal I: 1074.
• 8 Hampton J. R, and Mitchell J. R. A. 1966; b Abnormalities in platelet behaviour in acute illnesses. British Medical Journal 1: 1078.
• 9 Hampton J. R, and Mitchell J. R. A. 1966; c A transferable factor causing abnormal platelet behaviour in vascular disease. Lancet II: 764.
• 10 Hampton J. R, and Mitchell J. R. A. 1966; d Effect of glass contact on the electrophoretic mobility of human blood platelets. Nature (London) 209: 470.
• 11 Hampton J. R, Nicholls D. G, and Mitchell J. R. A. 1972; Evidence for two separate effects of adenosine diphosphate on human platelets. Thrombosis et Diathesis Haemorrhagica 28: 419.
• 12 Kerry G. P, and Taylor S. M. 1970; Effect of palmitate on human platelet glycolysis and aggregation. Proceedings of the Society for Experimental Biology and Medicine 135: 355.
• 13 McDonald L, and Edgill M. 1957; Coagulability of the blood in ischaemic heart disease. Lancet II: 457.
• 14 Mills D. C. B, and Roberts G. C. K. 1967; Membrane active drugs and the aggregation of human blood platelets. Nature (London) 213: 35.
• 15 Nicholls D. G, and Hampton J. R. 1972; Density Gradient separation of human platelets from plasma, and the role of plasma in adenosine diphosphate-induced platelet electrophoretic mobility changes. Thrombosis et Diathesis Haemorrhagica 27: 425.
• 16 O’Brien J. R, Heywood J. B, and Heady J. A. 1966; The quantitation of platelet aggregation by four compounds : a study in relation to myocardial infarction. Thrombosis et Diathesis Haemorrhagica 16: 752.
• 17 O’Brien J. R. 1961; The adhesiveness of native platelets and its prevention. Journal of Clinical Pathology 14: 140.
• 18 Seeman P. M. 1966; Membrane stabilisation by drugs, tranquilisers, steroids and anaesthetics. International Review of Neurobiology 9: 145.
• 19 Turpie A. G. G, McNicol G. P, and Douglas A. S. 1972; Platelet electrophoresis; effect of defibrination by ancrod (Arvin). Cardiovascular Research 6: 101.
• 20 Walsh P. N. 1972; Albumin density gradient separation and washing of platelets and the study of platelet coagulant activities. British Journal of Haematology 22: 205.