Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1973; 30(01): 173-177DOI: 10.1055/s-0038-1649114 Original Article Schattauer GmbH The Migration of Human Platelets in Vitro Paul Nathan 1 Shriners Burns Institute, Cincinnati Unit, and Departments of Physiology and Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio, U.S.A. › Author Affiliations Recommend Article Abstract Buy Article Summary A simple procedure is described for measuring migration of human platelets in vitro. Migration increased with temperature reaching maximum values at approximately 30 °C. Addition of normal plasma to the incubation chambers increased migration in all tests and the increase reached twice the control values in 3 of 4 tests. The rate of migration in vitro was relatively rapid for the first 12 hrs. of incubation and then continued at a slower rate for at least an additional 12 hours. Full Text References References 1 Deetjen H. 1901; Untersuchungen über die Blutplättchen. Virchow Archiv für Pathologische Anatomie und Physiologie und für Klinische Medizin 164: 239. 2 George M, and Vaughan J. H. 1962; In vitro cell migration as a model for delayed hypersensitivity. Proceedings of the Society for Experimental Biology and Medicine 111: 514. 3 Mackannes G.B. 1952; The action of drugs on intracellular tubercle bacilli. Journal of Pathology and Bacteriology 64: 429. 4 Lowenhaupt R. W, and Nathan P. 1968; Platelet accumulation observed by electron microscopy in the early phase of renal allotransplant rejection. Nature 220: 822. 5 Nathan P, and Lowenhaupt R. W. 1971; The effect of temperature on platelet migration in vitro. . Physiologist (abstract) 14: 200. 6 Nathan P. 1972; The effect of renal venous plasma from canine allotransplant recipients on platelet migration in vitro. . Proceedings of the Federation of American Societies for Experimental Biology 31: 234 (abstract).
