Glutaraldehyde Fixation of Human Blood Platelets

J. J Sixma; W. H Linssen; H. J Geuze

doi:10.1055/s-0038-1649366

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1972; 27(02): 272-277
DOI: 10.1055/s-0038-1649366

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Glutaraldehyde Fixation of Human Blood Platelets

Autor*innen

J. J Sixma

^*Division of Haemostasis and Thrombosis, State University, Utrecht, The Netherlands
W. H Linssen

^**Department of Internal Medicine and Center for Medical Electron Microscopy, State University, Utrecht, The Netherlands
H. J Geuze

^**Department of Internal Medicine and Center for Medical Electron Microscopy, State University, Utrecht, The Netherlands

Abstract

Summary

Hypertonic glutaraldehyde solutions produce dilatation of the surface connected system and shrinking of the cytoplasma. Hypertonic solutions produce swelling of the platelets. Good results are obtained with 1% glutaraldehyde M 0.07 M phosphate buffer (275 mosmol) 4 parts to 1 part platelet rich plasma. High glutaraldehyde concentrations do not affect the platelet ultrastructure.

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Referenzen

References
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