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Thromb Haemost 1996; 76(05): 697-702
DOI: 10.1055/s-0038-1650646
Original Article
Schattauer GmbH Stuttgart

Cloning of the Rat Tissue Factor cDNA and Promoter: Identification of a Serum-response Region

Olivier Taby
The Department of Medicine (Divisions of Cardiology and Thrombosis Research), Mount Sinai School of Medicine, New York, USA
,
Claire-Lise Rosenfield
The Department of Medicine (Divisions of Cardiology and Thrombosis Research), Mount Sinai School of Medicine, New York, USA
,
Vladimir Bogdanov
3   The Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, USA
,
Yale Nemerson
2   Department of Biochemistry, Mount Sinai School of Medicine, New York, USA
,
Mark B Taubman
1   The Cardiovascular Institute, Mount Sinai School of Medicine, New York, USA
3   The Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, USA
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Publikationsverlauf

Received 14. Februar 1996

Accepted after resubmission 30. Juli 1996

Publikationsdatum:
11. Juli 2018 (online)

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Summary

Tissue factor (TF) initiates coagulation and its expression in vascular smooth muscle cells (VSMC) likely plays a role in the propagation of arterial thrombosis. We report cloning the cDNA and proximal promoter region of the rat TF gene. While maintaining the general structure and organization of the TF molecule, there is a surprising divergence (≈ 18%) between the derived amino acid sequences of the rat and mouse TF. In contrast, there is striking similarity (90%) in the 5’ untranslated regions. High levels of basal promoter activity were seen in rat VSMC with constructs containing 106 bp of sequence downstream from the putative transcription start site and 426 to 103 bp of upstream sequence. Deletion of the sequence from −103 to −79, containing a single SP1 site, removed virtually all of the basal and serum-induced activity. Removal of the NFkB site or two additional upstream SP1 sites had little effect on serum responsiveness. Removal of the 5’ untranslated region abolished most of the basal activity of the TF promoter, suggesting that its high degree of conservation may be due to the presence of transcriptional elements critical for TF expression in rodent VSMC.

 

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