Activation of Factor X in Bovine Prothrombin Preparations^[*]

Summary

Chromatographic fractionation of factor X (autoprothrombin III) and factor Xa (autoprothrombin C), obtained from bovine prothrombin preparations, on amberlite IRC-50 demonstrates activation of factor X under the conditions of prothrombin activation. The increase in factor Xa protein during prothrombin activation is due to incorporation of a non-active protein into the fraction. Prothrombin Activating Derivative B (PAD-B) is activated by concentrated salt solutions and not by stypven.

• References

• 1 Marciniak E, Seegers W. H. Autoprothrombin C: A second enzyme from prothrombin. Canad. J. Biochem 40: 597 1962;
• 2 Landaburu R. H, Albado E. Factor X precursor in purified bovine prothrombin preparations. coagulation 02: 269 1969;
• 3 Landaburu R. H, Abdala O. E, Morrone M. J. Proteolytic enzymes derived from prothrombin. Can. J. Biochem 43: 417 1965;
• 4 Seegers W. H. The purification of prothrombin. Rec. Chem. Progr 13: 143 1952;
• 5 Landaburu R. H, Curtino J. A standard preparation of bovine fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg) 12: 64 1964;
• 6 Seegers W. H. Activation of purified prothrombin. Proc. Soc. Exp. Biol. (N.Y.) 72: 677 1949;
• 7 Seegers W. H, Cole E. R, Harmison G. R, Marciniak E. Purification and some properties of autoprothrombin C. Canad. J. Biochem 41: 1047 1963;
• 8 Landaburu R. H, Seegers W. H. Methods for the concentration of bovine Ac-globulin. Thrombos. Diathes. haemorrh. (Stuttg) 06: 435 1961;
• 9 Seegers W. H, Landaburu R. H. Purification of prothrombin and thrombin by chromatography on cellulose. Canad. J. Biochem 38: 1405 1960;
• 10 Seegers W. H, Smith H. P. Factors which influence the activity of purified thrombin. Amer. J. Physiol 137: 348 1942;
• 11 Reno R. S, Seegers W. H. Two stage procedure for the quantitative determination of autoprothrombin III concentration and some applications. Thrombos. Diathes. haemorrh. (Stuttg) 18: 198 1967;
• 12 Cole E. R, Marciniak E, Seegers W. H. Procedures for the quantitative determination of autoprothrombin C. Thrombos. Diathes. haemorrh. (Stuttg) 08: 434 1962;
• 13 Landaburu R. H, Lima F, Albado E. Activation characteristics of bovine prothrombin preparations. Brit. J. Haemat 17: 83 1969;
• 14 Lechner K. On the source of autoprothrombin C. Thrombos. Diathes. haemorrh. (Stuttg) Suppl. 17 259-1965
• 15 Marciniak E, Seegers W. H. Experiments with prothrombin, prethrombin, autoprothrombin III and plasmas with prothrombin related deficiencies. New Istanbul Contr. clin. Sci 08: 117 1965;
• 16 Seegers W. H. Basic enzymology of blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg) 14: 213 1965;