Activation of Factor X in Bovine Prothrombin Preparations^[*]

Summary

Chromatographic fractionation of factor X (autoprothrombin III) and factor Xa (autoprothrombin C), obtained from bovine prothrombin preparations, on amberlite IRC-50 demonstrates activation of factor X under the conditions of prothrombin activation. The increase in factor Xa protein during prothrombin activation is due to incorporation of a non-active protein into the fraction. Prothrombin Activating Derivative B (PAD-B) is activated by concentrated salt solutions and not by stypven.

^* This work was supported by the Consejo Nacional de Investigaciones Científicas y Técnicas.

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