Urokinase-Induced Fibrinolysis of 125I-Fibrin

Anne P. Ball; Eugene D. Day

doi:10.1055/s-0038-1654256

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1970; 24(03/04): 463-474
DOI: 10.1055/s-0038-1654256

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Urokinase-Induced Fibrinolysis of ¹²⁵I-Fibrin

Conditions for First-Order Activation Kinetics, Density-Gradient Separation of Split Products, and a Microassay^[*]

Anne P. Ball

¹Departments of Microbiology and Immunology and of Surgery, Duke University Medical Center Durham, N.C. 27706 (USA)

,

Eugene D. Day

¹Departments of Microbiology and Immunology and of Surgery, Duke University Medical Center Durham, N.C. 27706 (USA)

› Author Affiliations

Abstract

Summary

By means of the density-gradient separation of soluble fibrin split products from ¹²⁵I-labeled fibrin in 0.8-ml gradients, a micro-technique for the measurement of urokinase activation of plasminogen was developed. The conditions necessary to maintain first-order activation kinetics were obtained and incorporated into the micro-technique, thus providing a linear micro-assay of 0.1-ml volumes of urokinase in the range of 2.5-50.0 CTA units.

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References

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