The Evaluation of the Two-Stage Prothrombin Assay

H. C Hemker; P. W Hemker; K. v. d Torren; P. P Devilee; W. Th Hermens; E. A Loeliger

doi:10.1055/s-0038-1654328

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1971; 25(03): 545-554
DOI: 10.1055/s-0038-1654328

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

The Evaluation of the Two-Stage Prothrombin Assay

Authors

H. C Hemker

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
P. W Hemker

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
K. v. d Torren

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
P. P Devilee

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
W. Th Hermens

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
E. A Loeliger

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

Abstract

Summary

It is shown that (and why) the traditional ways of evaluating a thrombin generation curve do not give acceptable estimations of the prothrombin content of the sample. An alternative method is shown to meet the requirements better; it consists of drawing the thrombin generation curve on a semi-logarithmic graph, and extrapolating the descending slope to zero time. This gives a thrombin concentration representing the prothrombin concentration of the original sample.

Full Text

References

References
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