Thromb Haemost 1979; 42(04): 1261-1275
DOI: 10.1055/s-0038-1657021
Original Article
Schattauer GmbH Stuttgart

The Interaction Between Heparin and Plasmin on Amidolysis

Sukehiro Kohn
The Second Department of Medicine, Hokkaido University School of Medicine
,
Shoki Sakurama
The Second Department of Medicine, Hokkaido University School of Medicine
,
Tokiyo Morioka
The Second Department of Medicine, Hokkaido University School of Medicine
,
Yoshito Ito
The Second Department of Medicine, Hokkaido University School of Medicine
,
Taro Yasukouchi
The Second Department of Medicine, Hokkaido University School of Medicine
,
Shoichi Nakagawa
The Second Department of Medicine, Hokkaido University School of Medicine
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Publikationsverlauf

Received 18. März 1978

Accepted 18. Dezember 1978

Publikationsdatum:
23. August 2018 (online)

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Summary

The effect of the interaction between heparin and plasmin not only on fibrinolytic, caseinolytic and esterolytic activities but also on amidolytic activity, since plasmin has amidolytic or amidase activity, was investigated.

Following were the results obtained from these investigations:

1. Heparin enhanced amidolytic activity of a fixed level of plasmin which was prepared by activating plasminogen with urokinase within the range from 2 to 64 units/ml of the final concentration of heparin

2. Heparin also enhanced amidolytic activity of a fixed level of plasmin which was prepared by converting plasminogen with insolubilized urokinase.

3. Heparin did not enhance or inhibit fibrinolytic, caseinolytic and esterolytic activities of a fixed level of plasmin which was prepared by activating plasminogen with urokinase, in the fibrinolytic activity within the range from 0.032 to 125 units/ml, in the caseinolytic activity within the range from 0.0125 to 100 units/ml, and in the esterolytic activity within the range from 0.016 to 128 units/ml, of the final concentration of heparin respectively.