Urokinase Quality: Analysis of Different Preparations

W Rogatti; K Yokoigawa; T Kobayashi

doi:10.1055/s-0038-1661285

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 53(02): 245-248
DOI: 10.1055/s-0038-1661285

Original Article

Schattauer GmbH Stuttgart

Urokinase Quality: Analysis of Different Preparations

W Rogatti

¹The Research and Development, Schwarz Co. Ltd., Monheim, West Germany

,

K Yokoigawa

²The Research Department of Biological Chemistry, Wakamoto Pharmaceutical Co. Ltd., Tokyo, Japan

,

T Kobayashi

²The Research Department of Biological Chemistry, Wakamoto Pharmaceutical Co. Ltd., Tokyo, Japan

› Author Affiliations

Abstract

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Summary

Five commercially available German Urokinase (UK) preparations were examined for purity, fibrinolytic activity, and molecular weight composition. With the exception of one preparation (B) all samples showed comparable fibrinolytic activity (91-107% of the activity declared). Preparations A, D, and E contained almost exclusively high-molecular-weight UK (HMW-UK), i.e., 82-86%. However, preparation C (from kidney tissue cultures) contained 95% of low-molecular-weight-UK (LMW-UK), and sample B consisted of nearly equal amounts of both HMW-UK and LMW-UK (47:53). Purity criteria (coagulative and lysozyme activity, endotoxin content, protease activity, HB_S antigen) were fulfilled by preparation E, and, with certain restrictions, also by preparations A and C.

Keywords

Urokinase preparations - Quality control - High and low molecular weight urokinase - Fibrinolytic activity

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References

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