PDF herunterladen
CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2012; 02(02): 14-17
DOI: 10.1055/s-0040-1703564
Original Article

ROLE OF NITRIC OXIDE AND ARGINASE IN THE PATHOGENESIS OF ORAL CANCER

Nidarsh D. Hegde
1   Department of Oral and Maxillofacial Surgery, A.B. Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore - 575 018, India
,
Suchetha Kumari
2   Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University, Mangalore - 575 018, India
,
Mithra N. Hegde
3   Department of Conservative Dentistry and Endodontics, A.B. Shetty Memorial Institute of Dental Sciences, Mangalore - 575 018, India
,
Shilpa Shetty
4   Central Research Laboratory, Nitte University, Mangalore - 575 018, India.
› Institutsangaben
› Weitere Informationen
Auch verfügbar auf
  als PDF herunterladen Lizenzen und Reprints

Abstract

The aim of the present study is to evaluate the status of serum nitric oxide and arginase activity in oral cancerous condition. The study consisted of 80 individuals. The subjects were divided into normal individuals and individuals with oral cancer. Blood sample was collected from each subject and serum separated. Nitric oxide concentration was measured as total nitrates and nitrites by the Griess reaction method and arginase activity was estimated by diacetylmonoxime method.

The results of this study showed significantly increased levels of nitric oxide and arginase activity in oral cancerous condition when compared to normal individuals. From the study it can be concluded that during carcinogenesis and tumor progression, the level of nitric oxide and arginase activity increases. This could result from a generalized increased nitric oxide synthesis throughout the body of the cancer patient or reflect increased nitric oxide degradation promoted by oxidative stress.

Keywords

Nitric oxide - Arginase - Oral Cancer


Publikationsverlauf

Artikel online veröffentlicht:
02. März 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Shou-Yen Kao, Yu-Wei Chu, Ya-Wei Chen, Kuo-Wei Chang, Tsung-Yun Liu. Detection and Screening of Oral Cancer and Pre-cancerous Lesions. J Chin Med Assoc 2009; 72(5): 227-33.
  • 2 Jenkin DC, Charles IG, Thomson LL, Moss DW, Holmes LS, Baylis SA, et al. Role of nitric oxide in tumor growth. Proc Natl Acad Sci USA 1995; 92:4392–6.
  • 3 Patel RP, McAndrew J, Sellak H, White RC, Jo H, Freeman BA, et al. Biological aspects of reactive nitrogen species. Biochim Biophys Acta 1999; 1411:385–400.
  • 4 Hogg N, Singh RJ, Kalyanaraman B. The role of glutathione in the transport and catabolism of nitric oxide. FEBS Lett 1996; 382:223–8.
  • 5 Li RH, Hotchkiss JH. Potential genotoxicity of chronically elevated nitric oxide: a review. Mutat Res 1995; 339:73–89.
  • 6 Beevi, SS., Rasheed, AM., Geetha, A.Evaluation of oxidative stress and nitric oxide levels patients with oral cavity cancer.Jpn J Clin O ncol.pages 2002: 379-385.
  • 7 Gallo O., Masini E., Morbidelli L., Franchi A., Fini-Storchi., Vergari, W A and Ziche, M., (1998).Role of nitric oxide in angiogenesis and tumor progression in head and neck cancer. 1998; 90(24):587-596.
  • 8 Connelly,S., Macabeo-Ong, M.,Dekker N.,Jordan R.,Schmidt B.,Increased NO levels and iNOS over expression in oral squamous cell carcinoma, oral oncology, 41 (3):261-267.
  • 9 Fukumura, D., Kashiwagi, S., Jain, RK. The role of nitric oxide in tumor progression. Nature reviews cancer. 2006;6: 521-534.
  • 10 Park S, Lee S, Song S, Heo D, Park B, Lee D, et al., The Effect of Nitric Oxide on Cyclooxygenase-2 (Cox-2) Overexpression in Head and Neck Cancer Cell Lines. Int. J. Cancer: 107, 729–738.
  • 11 Kepka-Lenhart,D., Mistry, S.K., Wu, G and Morris Jr, M. Arginase: a limiting factor for nitric oxide and polyamine synthesis by activated macrophages. Am J Physiol regulatory integrative comp physiol 2000; 279(6):R2237-R2242.
  • 12 Chang, C I., Liao J. C and Kuo, L. Macrophage arginase promotes tumor cell growth and suppresses nitric oxide-mediated tumor Cytotoxicity. Cancer Res 2001; 61(3):1100-1106.
  • 13 Peter Popovic J., Herbert Zeh J, III and Juan Ochoa B. Arginine and Immunity. J of Nutrition 2007:1681-86.
  • 14 Bansal., Vishal Ochoa and Juan B. Arginase availability, arginase, and the immune response. Current Opinion in Clinical Nutrition and Metabolic Care 2003; 6(2):223-28.
  • 15 Li RH, Hotchkiss JH. Potential genotoxicity of chronically elevated nitric oxide: are view. MutatRes1995 ; 339:73-89.
  • 16 Hesse, M., Modolell, M., LaFlamme, A. C., Schito, M., Fuentes, J. M., Cheev er, A. W., Pearce, J and Wyan, T. A. Differential regulation of nitric oxide synthase-2 and arginase-1 by type1/type2 cytokines in vivo. J. Immunol 2001; 167(11):6533-6544.