RELATION BETWEEN SALIVARY AND SERUM VITAMIN C LEVELS AND DENTAL CARIES EXPERIENCE IN ADULTS - A BIOCHEMICAL STUDY

Mithra N. Hegde; Suchetha Kumari; Nidarsh D. Hegde; Shilpa S. Shetty

doi:10.1055/s-0040-1703698

Journal of Health and Allied Sciences NU, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2013; 03(04): 030-033
DOI: 10.1055/s-0040-1703698

Original Article

RELATION BETWEEN SALIVARY AND SERUM VITAMIN C LEVELS AND DENTAL CARIES EXPERIENCE IN ADULTS - A BIOCHEMICAL STUDY

Mithra N. Hegde

¹Senior Professor and HOD Department of Conservative Dentistry and Endodontics, K. S.Hegde Medical Academy, Nitte University, Deralakatte, Mangalor

,

Suchetha Kumari

²Professor, Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University, Deralakatte, Mangalor

,

Nidarsh D. Hegde

³Professor, Department of Oral and Maxillofacial Surgery, A. B. Shetty Memorial Institute of Dental Sciences,Deralakatte, Mangalor

,

Shilpa S. Shetty

⁴Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University, Deralakatte, Mangalore

› Institutsangaben

Abstract

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Abstract

The aim of this study was to estimate the vitamin C levels in saliva and serum of caries free and caries active adults and to correlate the vitamin C level with DMFT index (D=decayed, m=missing, f=filled, t=teeth) index. The present study included eighty healthy adults who were divided into four groups; Control, Group- I, Group II and Group- III with a DMFT index 0, <3, <10 and > 10 respectively. Saliva and serum samples were collected from all the four groups. The vitamin C of saliva and serum was estimated by dinitro phenyl hydrazine (DNPH) method. One-way ANOVA was used to compare the vitamin C levels of saliva and serum. Only differences with 'p'value <0.05 were considered statistically significant. Saliva and serum vitamin C level decreases with increase in caries activity and is statistically significant suggesting the powerful antioxidant property of vitamin C.

Keywords

Vitamin C - Saliva - Serum - Dental caries

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Referenzen

References
1 Mittal S, Bansal V, Garg S, Atreja G, Bansal S. The diagnostic role of Saliva — A Review. Clin Exp Dent. 2011; 3(4):314-20.
2 Rezaei A and Sariri R. Salivary Diagnosis of Periodontitis Status: A Review. Pharmacologyonline. 2011; 1:428-444.
3 Nagler R, Klien I, Zarzhersky N, Drigues N, Reznick A. Characterization of the differentiated antioxidant profile of human saliva. Free Radical Biology and Medicine. 2002; 32(3):268-77.
4 Chiappelli, Iribarren F, Prolo P. Salivary biomarkers in psychobiological medicine. Bioinformation. 2006;8(1): 331–334.
5 Edgar W and Mullane D. Saliva and oral health. British dental Association. 2004;3:32-49.
6 Humphrey S and Williamson R. A review of saliva: normal composistion, flow and function. J of prosthetic dentistry. 2001; 85(2), 162–168.
7 Kaufman E and Lamster I. Analysis of saliva for periodontal diagnosis—a review. Journal of clinical periodontology 2000; 27(7):453-465.
8 Hegde MN, Kumari S, Hegde ND, Moany A. Correlation between Total Antioxidant Level and Dental Caries in Adults - an Invivo Study. RJPBCS. 2011; 2(4):864-870.
9 Scully DV and Langley-Evans SC. Salivary antioxidants and periodontal disease status.Proc Nutr Soc. 2002; 61(1):137-43.
10 Northrop-Clewes CA, Thurnham DI. Monitoring micronutrients in cigarette smokers. Clin Chem Acta. 2007; 377(1-2):14-38.
11 Panda K, Chattopadhyay R, Chattopadhyay D, Chatterjee IB. Cigarette smoke-induced protein oxidation and proteolysis is exclusively caused by its tar phase: prevention by vitamin C. Toxicol Lett. 2001; 123(1): 21-32.
12 World Health Organization,oral health survey 1987: Basic methods, 3^rd Ed, Geneva.
13 Castelli A, Martorana GE, Frasea AM and Meucci E. Colorimetric detection of plasma vitamin C comparison between 2,4-Di nitrophenyl hydrazine and phosphotunstic acid. Methods Acta Vitaminol Enzymol. 1981; 3(2):103-110.
14 Hu S, Yu T, Xie Y, Yang Y, Li Y, Zhou X, et al., Discovery of oral ?uid biomarkers for human oral cancer by mass spectrometry. Cancer Genomics Proteomics 2007;4:55–64.
15 Kawas S,. Rahimb ZHA, Ferguson DB. Potential uses of human salivary protein and peptide analysis in the diagnosis of disease. Archives of oral biology. 2012;57:1-9.
16 Marquis RE. Oxygen metabolism, oxidative stress and acid-base physiology of dental plaque biofilms. J Ind Microbiol. 1995; 15(3): 198-207.
17 Bagchi K and Puri S. Free radical and antioxidants in health and disease. Eastern Med HJ. 1998; 4:350-360.
18 Baydaa A and Yas B. The relation of salivary antioxidants to dental caries among overweight and obese adult aged 30-40 year-old at textile factory in Mosul city. J Bagh College Dentistry. 23; 2011: 141-145.
19 Stahl W and Sies H. Antioxidant defense, Vitamin E and C and carotenoids. Diab. 1997; 46:514-516.
20 Yochum LA, Folsom AR, Kushi LA. Intake of antioxidant vitamins and risk of death from stroke in postmenopausal women. Am J Clin Nutr. 2000; 72:476-483.
21 Mithra.N. Hegde, Nidarsh Hegde, Suchetha Kumari, Vinod R. Jathanna.Correlation between vitamin c level and dentalcaries – a clinical study. Jcaesok. 2011;1(2):59-61.