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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2016; 06(02): 051-056
DOI: 10.1055/s-0040-1708641
Original Article

Incidence and Pattern of Bacterial Growth in Propofol Vial - An In vitro Study

U. S. Raveendra
1   Professor, Department of Microbiology, Department of Anaesthesiology, K.S.Hegde Medical Academy, Mangalore
,
Rashmi Soori
2   Assistant Professor, Department of Anaesthesiology, K.S.Hegde Medical Academy, Mangalore
,
Vimal Kumar Karnekar
3   Professor and Head, Department of Microbiology, K.S.Hegde Medical Academy, Mangalore
,
Swathi N. Nayak
4   Consultant, Department of Microbiology, Adarsha Hospital, Kundapur
,
Anand Bangera
5   Professor and Head, Department of Anaesthesiology, K.S.Hegde Medical Academy, Mangalore
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Abstract

Introduction- Propofol vials are often used in parts or are opened and left unattended. This has lead to blood stream infections, surgical site infections and acute febrile episodes. A prospective observational study was undertaken to know the incidence and pattern of bacterial growth in samples of Propofol in tropical climate.

Materials and methods- Samples were collected from vials of propofol of different brands, both with and without edetate at different time intervals with relation to room temperature. Each sample of 1ml were inoculated in Brain Heart Infusion (BHI) and incubated for 48hours. Presence of bacterial growth and their pattern were studied.

Statistical analysis used- Paired t test for categorical variables and for non categorical variables Levine's test and Pearson correlation. Results- Overall 42.26% of samples showed bacterial growth. The incidence was more in samples of propofol without edetate (43.75%) compared to samples with edetate (41.97 %). Most common organism was Staphylococcus aureus, followed by Enterococcus, Acinetobacter, Bacillus species, Pseudomonas and Staphylococcus citrus.

Conclusion- Propofol vial once opened favours bacterial colonisation and growth. Adding edetate to propofol has not shown much benefit in decreasing the incidence.

Keywords

Propofol - Contamination - Bacterial growth


Publication History

Received: 01 December 2015

Accepted: 14 May 2016

Article published online:
22 April 2020

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Thieme Medical and Scientific Publishers Private Ltd.
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