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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(04): 539-543
DOI: 10.1055/s-0040-1714760
Original Article

The Effectiveness of Three Irrigation Systems in the Enterococcus faecalis Reduction after Instrumentation with a Reciprocating Instrument

Karina Gonzalez Camara Fernandes
1   Department of Endodontics, Universidade Brasil, Brazil
,
Bruno Barciela da Silva
1   Department of Endodontics, Universidade Brasil, Brazil
,
Nilton César Boer
1   Department of Endodontics, Universidade Brasil, Brazil
,
Danilo Rodrigues Mandarini
1   Department of Endodontics, Universidade Brasil, Brazil
,
Lucieni Cristina Trovati Moreti
1   Department of Endodontics, Universidade Brasil, Brazil
,
Augusto Shoji Kato
2   Department of Endodontics, Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Campinas, SP, Brazil
,
Carlos Eduardo da Silveira Bueno
2   Department of Endodontics, Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Campinas, SP, Brazil
,
Ana Grasiela da Silva Limoeiro
3   Department of Endodontics, Faculdade de Ilhéus Bahia, Brazil
,
Sergio Luiz Pinheiro
4   Department of Endodontics, Postgraduate Program in Health Sciences, Pontifical Catholic University of Campinas (PUC-Campinas), Postgraduate Program in Health Sciences, Campinas, São Paulo, Brazil
,
Alexandre Sigrist De Martin
2   Department of Endodontics, Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Campinas, SP, Brazil
,
Carlos Eduardo Fontana
4   Department of Endodontics, Postgraduate Program in Health Sciences, Pontifical Catholic University of Campinas (PUC-Campinas), Postgraduate Program in Health Sciences, Campinas, São Paulo, Brazil
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Abstract

Objective This study aimed to analyze the effectiveness of three irrigation systems: EndoActivator, passive ultrasonic irrigation (PUI), and Easy Clean in the reduction of Enterococcus faecalis, after instrumentation with the reciprocating system, through microbiological collection and culture method.

Materials and Methods A total of 60 extracted human lower premolars were used and standardized at 16 mm in length. The teeth were accessed, contaminated with E. faecalis, and incubated for 21 days at 37°C. Initial collections (S1) were made with an absorbent paper cone to confirm the contamination; subsequently, instrumentation was performed with WaveOne Primary. The teeth were divided into four groups according to the final irrigation protocol (n = 15): group 1. EndoActivator; group 2, PUI; group 3, Easy Clean; and group 4, control group irrigated with saline solution sterile and without agitation. In the final irrigation, the agitation of the 17% ethylene amine tetra-acetic acid (EDTA) solutions was used, then 2.5% sodium hypochlorite (NaOCl); in both for this, three cycles of 20 seconds each. After the chemical–mechanical preparation and agitation of the irrigating solutions, the final collections (S2) for counting the colony-forming units (CFU/mL) occurred.

Results The Kruskal–Wallis test revealed that all the agitation systems reduced by 100% and the control group by 65.7%.

Conclusion The control group presented a significantly higher amount of CFU/mL after the chemical–mechanical preparation than the other groups, which were similar to each other (p > 0.05).

Keywords

endodontics - EndoActivator - Easy Clean - Enterococcus faecalis - PUI


Publication History

Article published online:
08 September 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
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