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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2020; 10(03): 109-115
DOI: 10.1055/s-0040-1715995
Original Article

Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

Shreya Udaya
1   Division of Molecular Genetics and Cancer, Nitte University Centre for Science and Education, Nitte (Deemed to be University), Deralakatte, Mangalore, Karnataka, India
,
Nishith Babu
1   Division of Molecular Genetics and Cancer, Nitte University Centre for Science and Education, Nitte (Deemed to be University), Deralakatte, Mangalore, Karnataka, India
,
Dechamma Pandyanda Nanjappa
1   Division of Molecular Genetics and Cancer, Nitte University Centre for Science and Education, Nitte (Deemed to be University), Deralakatte, Mangalore, Karnataka, India
,
Krithika Kalladka
1   Division of Molecular Genetics and Cancer, Nitte University Centre for Science and Education, Nitte (Deemed to be University), Deralakatte, Mangalore, Karnataka, India
,
Gunimala Chakraborty
1   Division of Molecular Genetics and Cancer, Nitte University Centre for Science and Education, Nitte (Deemed to be University), Deralakatte, Mangalore, Karnataka, India
,
Anirban Chakraborty
1   Division of Molecular Genetics and Cancer, Nitte University Centre for Science and Education, Nitte (Deemed to be University), Deralakatte, Mangalore, Karnataka, India
› Author Affiliations Funding The financial support from Nitte (Deemed to be University) through the intramural research grant (NUSR2/2018/10/56) to the first and the corresponding author is gratefully acknowledged.
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Abstract

Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish.

Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish.

Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark.

Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

Keywords

Cassia fistula stem bark - embryonic toxicity - ROS - zebrafish - natural antioxidant


Publication History

Article published online:
20 August 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
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