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Drug Res (Stuttg) 2016; 66(08): 420-426
DOI: 10.1055/s-0042-108186
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Reversible Anti-Spermatogenic Effect of Piperine on Epididymis and Seminal Vesicles of Albino Rats

G. Chinta
1   DBT-Interdisciplinary Program in Life Sciences, Pondicherry University, Puducherry, India
,
L. Periyasamy
2   Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, India
› Institutsangaben
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Publikationsverlauf

received 29. März 2016

accepted 02. Mai 2016

Publikationsdatum:
09. Juni 2016 (online)

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Abstract

Background: We have recently proved the interactions of piperine with androgen receptor and androgen binding protein. The present study was aimed to evaluate the antifertility effect of piperine on male albino rats after the treatment period i. e., after 60 days and withdrawal period i. e., after 120 days.

Materials and Methods: Adult male rats were divided into 4 groups (n=12). Group I: Control: Rats were given vehicle p.o i. e., 0.5% carboxy methyl cellulose (CMC) in normal saline daily for 60 days, Group II: Rats were treated with piperine suspended in 0.5% CMC at a dose of 10 mg/kg daily/60 days. Group III: Rats were treated with piperine suspended in 0.5% CMC at a dose of 10 mg/kg on every 4th day for 60 days. Group IV: Rats were treated with piperine suspended in 0.5% CMC at a dose of 10 mg/kg on every 7th day for 60 days.

Results: Piperine significantly altered the epididymal sperm count, motility, viability, weight of the epididymis, cauda, caput, corpus and seminal vesicles. It also exhibited negative impact on biochemical markers via decreasing epididymal sialic acid levels, seminal fructose content, epididymal anti-oxidant enzyme activities of super oxide dismutase (SOD), catalase (CAT) and by increasing the malondialdehyde content after the treatment period. Histopathological observations also supported the above findings. All the altered values were reinforced after the withdrawal period.

Conclusion: From the results of this study, we can conclude that piperine has the potential to become a good lead for the reversible male oral contraceptive research.

Key words

piperine - reversible male oral contraceptive - anti-fertility activity - Withdrawal period - Anti-oxidant enzymes - Sialic acid levels
 

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