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CC BY 4.0 · Eur J Dent 2023; 17(03): 797-803
DOI: 10.1055/s-0042-1755624
Original Article

Effects of Chrysin on Oral Squamous Cell Carcinoma In Vitro

Duangchewan Puengsurin
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
,
Supranee Buranapraditkun 
2   Division of Allergy and Clinical Immunology, Department of Medicine, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Thai Red Cross Society, Bangkok 10330, Thailand
3   Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center-Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
4   Thai Pediatric Gastroenterology, Hepatology and Immunology (TPGHAI) Research Unit, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, The Thai Red Cross Society, Bangkok 10330, Thailand
,
Chayanee Leewansangtong
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
,
Nitchakarn Taechaaukarakul
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
,
Proud Songsivilai
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
,
Rudee Surarit 
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
,
Nakarin Kitkumthorn
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
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Abstract

Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural product. Previous research on chrysin's biological functions, including anticancer activity, had been reported. However, chrysin's effect on oral squamous cell carcinoma (OSCC) is still scarce. This article aimed to test the cytotoxicity, antiproliferative, antimigration, anti-invasion, and apoptotic effects of purified chrysin in two OSCC cell lines, HSC4 and SCC25.

Materials and Methods The malignant phenotype was assessed using cell proliferation, wound healing, and transwell assays. Cell apoptosis was determined using flow cytometry. The positive control was OSCC cells treated with cisplatin, and the negative control was OSCC cells incubated with 0.1% dimethyl sulfoxide.

Results Chrysin at concentrations of 100 and 200 µM could inhibit OSCC cell proliferation, migration, and invasion, as well as enhance cell apoptosis, particularly in the early stages of apoptosis.

Conclusion In OSCC cell lines, chrysin has been demonstrated to be an effective antioncogenic agent. Additional research is required to confirm the results. Chrysin should be suggested as a possible alternative therapeutic application for OSCC.

Keywords

chrysin - oral squamous cell carcinoma - proliferation - migration - invasion - apoptosis

Supplementary Material



Publication History

Article published online:
27 September 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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