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CC BY-NC-ND 4.0 · Indian J Plast Surg 2023; 56(01): 053-061
DOI: 10.1055/s-0042-1759495
Original Article

The Effect of Centrifuge Duration on Fat Graft Survival

Oguzhan Demirel 
1   Plastic, Rekonsturctive and Aesthetic Surgery Department, Istanbul Aydın University Faculty of Medicine, Istanbul, Turkey
2   Department of Plastic, Reconstructive, and Aesthetic Surgery, Bagcılar Research and Training Hospital, Istanbul, Turkey
,
Percin Karakol 
2   Department of Plastic, Reconstructive, and Aesthetic Surgery, Bagcılar Research and Training Hospital, Istanbul, Turkey
,
Mehmet Bozkurt
2   Department of Plastic, Reconstructive, and Aesthetic Surgery, Bagcılar Research and Training Hospital, Istanbul, Turkey
,
Mehmet Ibrahim Tuglu 
3   Department of Histology-Embriyology, Celal Bayar University Medical Faculty, Manisa, Turkey
› Author Affiliations Funding Financial support was received from the Unıversıty of Health Scıences Scientific Research Projects Unit for the supply of experimental animals in this study.
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Abstract

Background Although fat grafts are widely used for reconstruction and aesthetic purposes, their survival rates differ significantly. Centrifugation is one of the methods used to increase the survival of fat grafts. However, experimental studies examining the long-term outcomes of centrifugation duration are currently limited. Thus, in the present study, the effects of centrifugation duration on the survival of fat grafts were assessed using an animal model.

Methods Thirty Sprague Dawley rats were included in the study and fat grafts were obtained from each specimen by excision from inguinal fat pads. Preparation protocols were administered as an en-bloc fat graft in Group 1, minced fat graft in Group 2, and fat graft centrifuged at 1,054 ×g for 2 minutes, 3 minutes, and 4 minutes in Group 3, 4, and 5, respectively. After 12 weeks of follow-up, grafts were harvested and were subjected to histopathological evaluation based on an established scoring system.

Results En-block fat grafts were associated with necrosis, fibrosis, inflammation, vacuole formation, and alterations in adipocyte morphology. Among the three centrifugation groups, Group 3 demonstrated the best adipocyte viability and vascularity. However, graft weights decreased in all experimental groups.

Conclusion The centrifugation process may have positive effects on adipocyte survival by means of purifying the fat graft and increasing adipocyte concentration. When the centrifugal durations were compared, 3-minute centrifuge yielded the most favorable results.

Keywords

adipose - centrifugation - graft - duration - survival

Ethical Approval

This study was conducted under the permission of the Health Sciences University Bagcılar Training and Research Hospital Local Ethical Committee. All applicable international, national, and institutional guidelines for the care and use of animals were followed.




Publication History

Article published online:
22 December 2022

© 2022. Association of Plastic Surgeons of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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