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CC BY 4.0 · Journal of Health and Allied Sciences NU 2024; 14(S 01): S76-S85
DOI: 10.1055/s-0044-1786694
Original Article

Neuroprotective Potential of Hygrophila auriculata Targeting Oxidative Stress-Mediated Deficits in Streptozotocin-Induced Sciatic Nerve Injury

Autor*innen

  • Vishal B. Jadhav 

    1   Department of Pharmacology, School of Pharmaceutical Sciences, Sandip University, Nashik, Maharashtra, India
    2   Department of Pharmacology, Bhupal Nobles' College of Pharmacy, Bhupal Nobles' University, Udaipur, Rajasthan, India

  • Jai Singh Vaghela 

    2   Department of Pharmacology, Bhupal Nobles' College of Pharmacy, Bhupal Nobles' University, Udaipur, Rajasthan, India

Funding None.
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Abstract

Objective Diabetic neuropathy, a microvascular complication of diabetes, affects 50% of individuals. Addressing this challenge is challenging due to its poorly understood origin and existing therapeutic approaches. This study used a methanolic extract from Hygrophila auriculata (MEHA) to treat oxidative stress-induced sciatic nerve injury in diabetic rats.

Materials and Methods A study was conducted to assess the nociceptive reflex after a single streptozotocin (STZ) (45 mg/kg intraperitoneal.) injection. The rats were divided into six groups (n = 6 rats per group). Group I nondiabetic (ND) rats received oral gavage of 1% carboxymethyl cellulose (CMC). The diabetic rats in groups II to VI were given 1% CMC, 100, 200, and 400 mg/kg of MEHA, and 180 mg/kg of metformin (MET). The freshly prepared 1% (w/v) CMC suspension of both MEHA and MET was administered over a 4-week period, commencing from the 28th day through the 56th day post-STZ injection. The impact of STZ-induced sciatic nerve injury was analyzed through the estimation of serum glucose and glycohemoglobin levels, paw withdrawal and tail-flick latencies, oxidative stress markers, and neural histoarchitecture.

Results Diabetic (STZ) control group II showed significantly altered serum glucose and glycohemoglobin levels, a reduced paw withdrawal threshold, and reduced paw withdrawal and tail-flick latencies in contrast to ND group I. Furthermore, increased oxidative stress in the sciatic nerve correlates with a reduced nociceptive threshold and disrupted neural histoarchitecture in diabetic rats. These behavioral, biochemical, and molecular changes were markedly and dose-dependently reduced by MEHA and MET treatments.

Conclusion The antioxidant efficacy of MEHA modulated oxidative stress in STZ-sensitized diabetic rats and corrected neuropathic pain by attenuating hyperglycemia.

Keywords

hyperalgesia - allodynia - diabetic neuropathy - Hygrophila auriculata - oxidative stress

Ethical Approval

Approval for the experiment's protocol was granted by the Institutional Animal Ethics Committee (IAEC) of Bhupal Nobles' College of Pharmacy, located in Udaipur, Rajasthan, India (Approval Number: 870/PO/Re/S/05/CPCSEA).


Availability of Data and Materials

All data generated or analyzed during this study are included in this published article.


Code Availability

Not applicable


Authors' Contributions

V.B.J. and J.S.V formulated the conceptualization and thematic framework of the study V.B.J. conducted and compiled the experimental studies, while the overall results were jointly analyzed by V.B.J. and J.S.V. V.B.J. took the lead in drafting the manuscript under the guidance of J.S.V. Both authors have thoroughly reviewed and given approval for the manuscript.




Publikationsverlauf

Artikel online veröffentlicht:
29. April 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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