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DOI: 10.1055/s-0044-1787848
Compromised Differentiation Potential of Diabetic Dental Pulp Stem Cells
Funding This study was funded by Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune, Maharashtra, India [DPU927(22)2018].
Abstract
Background Dental pulp-derived mesenchymal stem cells (DPSCs) are documented to be a promising source for the treatment of a diverse spectrum of diseases including type 2 diabetes mellitus (T2DM). However, alterations in the characteristics of DPSCs from the T2DM patients are still unclear.
Objective The purpose of this study was to compare the characteristics of dental pulp stem cells obtained from diabetic and nondiabetic healthy individuals.
Methods Dental pulp stem cells from nondiabetic (ND-DPSCs) and diabetic (D-DPSCs) were isolated by the explant culture method. Both cells were expanded in identical culture conditions and subsequently differentiated into osteogenic, chondrogenic, and adipogenic conditions. D-DPSCs and ND-DPSCs were characterized for a panel of MSCs-specific surface markers. Senescence associated with β-galactosidase was performed. In addition, we also performed an in vivo chick embryo yolk sac membrane assay for angiogenesis.
Results Findings of this study showed that diabetes mellitus affected the osteogenic and chondrogenic differentiation, while adipogenic differentiation was significantly higher in D-DPSCs as compared to ND-DPSCs. Clonogenic ability and angiogenic potential of ND-DPSCs is higher than D-DPSCs despite similar surface marker expressions.
Conclusion Diabetes affects the stemness of D-DPSCs in terms of clonogenic, osteogenic, and chondrogenic differentiation and angiogenic potential, reflecting the adverse effects of hyperglycemia even on dental pulp stem cells.
# These authors have contributed equally.
Publikationsverlauf
Artikel online veröffentlicht:
20. Juni 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
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References
- 1 Tabish SA. Is diabetes becoming the biggest epidemic of the twenty-first century?. Int J Health Sci (Qassim) 2007; 1 (02) V-VIII
- 2 Galicia-Garcia U, Benito-Vicente A, Jebari S. et al. Pathophysiology of type 2 diabetes mellitus. Int J Mol Sci 2020; 21 (17) 1-34
- 3 Chaudhury A, Duvoor C, Reddy Dendi VS. et al. Clinical review of antidiabetic drugs: implications for type 2 diabetes mellitus management. Front Endocrinol (Lausanne) 2017; 8 (January): 6
- 4 Rodríguez-Fuentes DE, Fernández-Garza LE, Samia-Meza JA, Barrera-Barrera SA, Caplan AI, Barrera-Saldaña HA. Mesenchymal stem cells current clinical applications: a systematic review. Arch Med Res 2021; 52 (01) 93-101
- 5 Päth G, Perakakis N, Mantzoros CS, Seufert J. Stem cells in the treatment of diabetes mellitus - Focus on mesenchymal stem cells. Metabolism 2019; 90: 1-15
- 6 Patil VR, Kharat AH, Kulkarni DG, Kheur SM, Bhonde RR. Long term explant culture for harvesting homogeneous population of human dental pulp stem cells. Cell Biol Int 2018; 42 (12) 1602-1610
- 7 Sanap A, Bhonde R, Joshi K. Conditioned medium of adipose derived mesenchymal stem cells reverse insulin resistance through downregulation of stress induced serine kinases. Eur J Pharmacol 2020; 881: 173215
- 8 As MN, Deshpande R, Kale VP, Bhonde RR, Datar SP. Establishment of an in ovo chick embryo yolk sac membrane (YSM) assay for pilot screening of potential angiogenic and anti-angiogenic agents. Cell Biol Int 2018; 42 (11) 1474-1483
- 9 Solis MA, Moreno Velásquez I, Correa R, Huang LLH. Stem cells as a potential therapy for diabetes mellitus: a call-to-action in Latin America. Diabetol Metab Syndr 2019; 11 (01) 20
- 10 Chen S, Du K, Zou C. Current progress in stem cell therapy for type 1 diabetes mellitus. Stem Cell Res Ther 2020; 11 (01) 275
- 11 Baer PC, Geiger H. Adipose-derived mesenchymal stromal/stem cells: tissue localization, characterization, and heterogeneity. Stem Cells Int 2012; 2012: 812693
- 12 Suchánek J, Nasry SA, Soukup T. The differentiation potential of human natal dental pulp stem cells into insulin-producing cells. Folia Biol (Praha) 2017; 63 (04) 132-138
- 13 Xu B, Fan D, Zhao Y. et al. Three-dimensional culture promotes the differentiation of human dental pulp mesenchymal stem cells into insulin-producing cells for improving the diabetes therapy. Front Pharmacol 2020; 10 (January): 1576
- 14 Catanzaro O, Dziubecki D, Lauria LC, Ceron CM, Rodriguez RR. Diabetes and its effects on dental pulp. J Oral Sci 2006; 48 (04) 195-199
- 15 Shree N, Bhonde RR. Metformin preconditioned adipose derived mesenchymal stem cells is a better option for the reversal of diabetes upon transplantation. Biomed Pharmacother 2016; 84: 1662-1667
- 16 Kravchuk E, Grineva E, Bairamov A, Galagudza M, Vlasov T. The effect of metformin on the myocardial tolerance to ischemia-reperfusion injury in the rat model of diabetes mellitus type II. Exp Diabetes Res 2011; 2011: 907496
- 17 Samiei M, Aghazadeh M, Movassaghpour AA. et al. Isolation and characterization of dental pulp stem cells from primary and permanent teeth. J Am Sci 2013; 9 (12) 153-157
- 18 Yu S, Li J, Zhao Y, Li X, Ge L. Comparative secretome analysis of mesenchymal stem cells from dental apical papilla and bone marrow during early odonto / osteogenic differentiation: potential role of transforming growth factor- β 2. Front Physiol 2020; 11 (March): 1-14
- 19 Efimenko AY, Kochegura TN, Akopyan ZA, Parfyonova YV. Autologous stem cell therapy: how aging and chronic diseases affect stem and progenitor cells. Biores Open Access 2015; 4 (01) 26-38
- 20 Phadnis SM, Ghaskadbi SM, Hardikar AA, Bhonde RR. Mesenchymal stem cells derived from bone marrow of diabetic patients portrait unique markers influenced by the diabetic microenvironment. Rev Diabet Stud 2009; 6 (04) 260-270
- 21 Mahmoud M, Abu-Shahba N, Azmy O, El-Badri N. Impact of diabetes mellitus on human mesenchymal stromal cell biology and functionality: implications for autologous transplantation. Stem Cell Rev Rep 2019; 15 (02) 194-217
- 22 Cassidy FC, Shortiss C, Murphy CG. et al. Impact of type 2 diabetes mellitus on human bone marrow stromal cell number and phenotypic characteristics. Int J Mol Sci 2020; 21 (07) 1-20
- 23 Marin C, Luyten FP, Van der Schueren B, Kerckhofs G, Vandamme K. The impact of type 2 diabetes on bone fracture healing. Front Endocrinol (Lausanne) 2018; 9 (01) 6