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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0044-1791937
Original Article

Moringa oleifera L. Nanosuspension Extract Administration Affects Heat Shock Protein-10 and -70 under Orthodontics Mechanical Force In Vivo

Ari Triwardhani
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Alida Alida
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ervina Restiwulan Winoto
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Adya Pramusita
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nurul Aisyah Rizki Putranti
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Kristian Satrio Ariadi
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Okso Brillian Pribadi
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Amelia Aisyiah Anwar
2   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Alqomariyah Eka Purnamasari
2   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rifqah Ananda Mappananrang
2   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Putri Cahaya Situmorang
3   Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
,
Wibi Riawan 
4   Department of Molecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
,
Tengku Natasha Eleena binti Tengku Ahmad Noor
5   Membership of Faculty of Dental Surgery, Royal Collage of Surgeon, Edinburgh University, United Kingdom
,
Albertus Putera Nugraha 
6   Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
,
Alexander Patera Nugraha
1   Department of Orthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
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Abstract

Objective The mechanical stimulation known as orthodontic mechanical force (OMF) causes biological reactions in orthodontic tooth movement (OTM). Heat shock protein-70 (HSP-70) needs pro-inflammatory cytokines to trigger bone resorption in OTM; nevertheless, heat shock protein-10 (HSP-10), a “Alarmin” cytokine, should control these pro-inflammatory cytokines to get the best alveolar bone remodeling (ABR). Moringa oleifera L. nanosuspension extract (MONE) has anti-inflammatory, antioxidant, and ABR-stimulating properties. The aim of the study was to examine in vivo HSP-10 and HSP-70 expressions under OMF following MONE application in Wistar rats (Rattus norvegicus).

Material and Methods A total of 36 Wistar rats (R. norvegicus) were split up into eight groups: one for treatment (OMF + MONE) and one for control (OMF + MONE administration for days 1, 7, 14, and 21). By employing nickel–titanium coil springs and using 10 g of light force per millimeter to implant the orthodontic device, the OMF was completed. According to the day of observation, all of the samples were sacrificed. To perform an immunohistochemistry investigation, the premaxilla of the sample was isolated. Tukey's Honest Significant Different (HSD) test (p < 0.05) was performed after an Analysis of Variance (ANOVA) analysis of the data.

Results In both the OMF and MONE groups, HSP-70 peaked on day 14 and began to fall on day 21. HSP-10 peaked on day 21, but along with MONE, it also began to progressively decline on days 14 and 21, with significant differences (p < 0.05).

Conclusion According to immunohistochemistry evidence, postadministration of MONE markedly elevated HSP-10 but lowered HSP-70 expression in the alveolar bone of Wistar rats under OMF.

Keywords

dentistry - heat shock proteins - medicine - Moringa oleifera - orthodontics


Publication History

Article published online:
09 January 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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