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CC BY 4.0 · Journal of Health and Allied Sciences NU
DOI: 10.1055/s-0044-1791966
Original Article

The Effect of CLA Supplementation and High-Intensity Interval Training on Plasma Neuregulin 4, Interleukin 6, and TNF-α in Obese Men

Asieh Abbassi-Daloii
1   Department of Sport Physiology, Faculty of Sport, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
,
Alireza Barari
1   Department of Sport Physiology, Faculty of Sport, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
,
Niloufar Bolboli
1   Department of Sport Physiology, Faculty of Sport, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
,
Ayoub Saeidi
2   Department of Sport, Faculty of Physical Education and Sport Sciences, University of Kurdistan, Kurdistan, Iran
,
Ghadir Zahabi 
3   Department of Sport, Faculty of Sport and Physical Education, University of Belgrade, Belgrade, Serbia
,
Amador García-Ramos 
4   Department of Physical Education and Sport, Faculty of Sport Sciences, University of Granada, Granada, Spain
5   Department of Sports Sciences and Physical Conditioning, Faculty of Education, Universidad Católica de la Santísima Concepción, Concepción, Chile
,
Vladimir Ilic 
3   Department of Sport, Faculty of Sport and Physical Education, University of Belgrade, Belgrade, Serbia
,
Aleksandar Nedeljkovic
3   Department of Sport, Faculty of Sport and Physical Education, University of Belgrade, Belgrade, Serbia
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Abstract

Background Despite extensive research, the effects of conjugated linoleic acid (CLA) supplementation in treating obesity are still unclear and equivocal. Adipokines play an important regulatory role in the activation of adipose tissue function. The purpose of the present research is to investigate the efficacy of CLA supplementation during high-intensity interval training (HIIT) on plasma neuregulin 4 (NRG-4), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) in obese men.

Methodology Forty-four nonactive, obese males (age = 26.29 ± 0.97 y, body mass index [BMI] = 32.29 ± 1.27 kg/m2) participated in this research. Participants were randomly divided into four groups: control, without training or CLA supplementation; supplement, CLA supplementation without training; exercise, HIIT without CLA supplementation; supplement + exercise, HIIT with CLA supplementation. HIIT was performed for 12 weeks, three sessions per week, with a training intensity ranging from 60 to 75% of the VO2 max on a treadmill. Subjects in the CLA supplement groups consumed two 1-g CLA capsules daily in two meals with breakfast and dinner. The amount of plasma NRG-4, IL-6, and TNF-α was measured using an ELISA (enzyme-linked immunosorbent assay) kit. Various variables were collected at two time points (pretest and posttest).

Results The study found that both the HIIT and HIIT + CLA groups showed greater improvements in weight and BMI compared with the other groups. Notably, posttest levels of NRG-4 were significantly higher in the CLA, HIIT, and HIIT + CLA groups compared with the control group (p < 0.001). Additionally, following the intervention period, IL-6 levels in the HIIT + CLA group and TNF-α levels in the HIIT and HIIT + CLA groups were significantly lower than in the control group (p < 0.001).

Conclusion CLA supplementation, in conjunction with HIIT, emerges as a highly effective approach and more benefits on the amount of adipokines secreted from fat tissue in obese people than either of them separately.

Keywords

conjugated linoleic acid - immune functions - therapeutic window - obesity-related inflammation


Publication History

Article published online:
07 November 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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