Molecular Phylogenetics of Bromeliaceae: Evidence from trnL(UAA) Intron Sequences of the Chloroplast Genome

R. Horres; G. Zizka; G. Kahl; K. Weising

doi:10.1055/s-2000-3700

Plant Biology, Inhaltsverzeichnis

Plant Biol (Stuttg) 2000; 2(3): 306-315
DOI: 10.1055/s-2000-3700

Original Paper

Georg Thieme Verlag Stuttgart ·New York

Molecular Phylogenetics of Bromeliaceae: Evidence from trnL(UAA) Intron Sequences of the Chloroplast Genome

R. Horres ¹ , G. Zizka ¹ , G. Kahl ² , K. Weising ³

¹ Botany/Paleobotany, Forschungsinstitut Senckenberg and Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt/M., Germany
² Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt/M., Germany
³ Plant Morphology and Systematics, Universität Kassel, Kassel, Germany

Abstract

Phylogenetic relationships within Bromeliaceae were assessed by comparative sequencing of chloroplast trnL(UAA) intronic DNA. Sixty-seven taxa were analyzed, encompassing 62 species, two subspecies and two varieties from 32 bromeliad genera. One representative of Rapateaceae and two of Velloziaceae were used as outgroups. Introns were amplified by the polymerase chain reaction and conserved flanking primers. Amplification products were sequenced directly. Of 700 base pairs total trnL (UAA) intron size, 551 bp could be unambiguously aligned. Sequence divergence among ingroup taxa ranged from zero to 4.6 %. Phylogenetic reconstruction by parsimony analysis of 73 informative base substitutions yielded 1092 most parsimonious trees. The topology of the resulting strict consensus tree was only partially consistent with current classification schemes. Brocchinia and Ayensua grouped close together and formed a well-supported basal clade, suggesting a sister group relationship of both to the remainder of the family. The remaining ingroup species were divided into an unresolved polytomy of three lineages, comprising (1) all members of subfamily Tillandsioideae, (2) the three investigated Hechtia species, and (3) all Bromelioideae and the remaining Pitcairnioideae (except Brocchinia, Ayensua and Hechtia). The latter clade was split into another polytomy of five sublineages, one of which comprised all Bromelioideae. Whereas monophyly of Tillandsioideae and Bromelioideae was supported by trnL intron data, Pitcairnioideae as traditionally circumscribed are clearly polyphyletic. There is evidence that the genera Tillandsia, Vriesea, Guzmania, Abromeitiella, Deuterocohnia, Dyckia, Pitcairnia and Aechmea in the present circumscription are not monophyletic.

Key words

Bromeliaceae - phylogeny - subfamilial grouping - delimitation of genera - trnL intron - chloroplast genome

Volltext

Referenzen

References

01 APG, . (1998); An ordinal classification for the families of flowering plants. Ann. Missouri Bot. Gard.. 85 531-553
02 Baldwin, B. G.,, Sanderson, M. J.,, Porter, J. M.,, Wojciechowski, M. F.,, Campbell, C. S.,, and Donghue, M. J.. (1995); The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Missouri Bot. Gard.. 82 242-277
03 Böhle, U. R.,, Hilger, H.,, and Martin, W. F.. (1996); Island colonization and evolution of the insular woody habit in Echium L. (Boraginaceae). Proc. Natl. Acad. Sci. USA. 93 11 740-11 745
04 Brown, G. K., and Gilmartin, A. J.. (1989 a); Chromosome numbers in Bromeliaceae. Amer. J. Bot.. 76 657-665
05 Brown, G. K., and Gilmartin, A. J.. (1989 b); Stigma types in Bromeliaceae. A systematic survey. Syst. Bot.. 14 110-132
06 Brown, G. K., and Terry, R. G.. (1992); Petal appendages in Bromeliaceae. Amer. J. Bot.. 79 1051-1071
07 Chase, M. W.,, Stevenson, D. W.,, Wilkin, P.,, and Ruddall, P. J.. (1995) Monocot systematics: a combined analysis. Monocotyledons: Systematics and Evolution. Rudall, P. J., Cribb, P. J., Cutler, D. F., and Humphries, C. J., eds. Kew; Royal Botanic Gardens pp. 685-730
08 Clark, W. D., and Clegg, M. T.. (1990); Phylogenetic comparisons among rbcL sequences in the Bromeliaceae. Amer. J. Bot.. 77 115 (Abstract)
09 Clark, W. D.,, Gaut, B. S.,, Duvall, M. R.,, and Clegg, M. T.. (1993); Phylogenetic relationships of the Bromeliiflorae - Commeliniflorae - Zingiberiflorae complex of monocots based on rbcL sequence comparisons. Ann. Missouri Bot. Gard.. 80 987-998
10 Cros, J.,, Combes, M. C.,, Trouslot, P.,, Anthony, F.,, Hamon, S.,, Charrier, A.,, and Lashermes, P.. (1998); Phylogenetic analysis of chloroplast DNA variation in Coffea L. Mol. Phylogenet. Evol.. 9 109-117
11 Doyle, J. J., and Doyle, J. L.. (1987); A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull.. 19 11-15
12 Duvall, M. R.,, Clegg, M. T.,, Chase, M. W.,, Clark, W. D.,, Kress, W. J.,, Hills, H. G.,, Eguiarte, L. E.,, Smith, J. F.,, Gaut, B. S.,, Zimmer, E. A.,, and Learn jr., G. H.. (1993); Phylogenetic hypotheses for the monocotyledons constructed from rbcL sequence data. Ann. Missouri Bot. Gard.. 80 607-619
13 Eriksson, T.. (1998) AutoDecay, version 4.0. Stockholm University; Program available from the author at http://www.bergianska.se/personal/TorstenE/AutoDecay/
14 Felsenstein, J.. (1985); Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 39 783-791
15 Felsenstein, J.. (1995) PHYLIP 3.5 manual. Berkeley; University of California Herbarium
16 Gardner, C. S.. (1986); Preliminary classification of Tillandsia based on floral characters. Selbyana. 9 130-146
17 Gielly, L., and Taberlet, P.. (1996); A phylogeny of the European gentians inferred from chloroplast trnL (UAA) intron sequences. Bot. J. Linn. Soc.. 120 57-75
18 Gilmartin, A. J., and Brown, G. K.. (1987); Bromeliales, related monocots, and resolution of relationships among Bromeliaceae subfamilies. Syst. Bot.. 12 493-500
19 Gilmartin, A. J.,, Brown, G. K.,, Varadarajan, G. S.,, and Neighbors, N.. (1989); Status of Glomeropitcairnia within evolutionary history of Bromeliaceae. Syst. Bot.. 14 339-348
20 Givnish, T. J.,, Sytsma, K. J.,, and Smith, J. F.. (1990); A re-examination of phylogenetic relationships among bromeliad subfamilies using cpDNA restriction site variation. Amer. J. Bot.. 77 133 (Abstract)
21 Givnish, T. J.,, Sytsma, K. J.,, Smith, J. F.,, Hahn, W. J.,, Benzing, D. H.,, and Burkhardt, E. N.. (1997) Molecular evolution and adaptive radiation in Brocchinia (Bromeliaceae: Pitcairnioideae) atop the Tepuis of the Guyana Shield. Molecular Evolution and Adaptive Radiation. Givnish, T .J., Sytsma, K. J., eds. Cambridge, UK; Cambridge University Press pp. 259-311
22 Givnish, T. J.,, Evans, T. M.,, Pires, J. C.,, and Sytsma, K. J.. (1999); Polyphyly and convergent morphological evolution in Commelinales and Commelinidae: Evidence from rbcL sequence data. Mol. Phylogenet. Evol.. 12 360-385
23 Grant, J. R.. (1993); True Tillandsias misplaced in Vriesea (Bromeliaceae: Tillandsioideae). Phytologia. 75 170-175
24 Grant, J. R.. (1995); The resurrection of Alcantarea and Werauhia, a new genus (Bromeliaceae: Tillandsioideae). Trop. subtrop. Pflanzenwelt. 91 1-57
25 Gross, E.. (1988); Bromelienstudien IV. Zur Morphologie der Bromeliaceen-Samen unter Berücksichtigung systematisch-taxonomischer Aspekte. Trop. subtrop. Pflanzenwelt. 64 412-625
26 Halbritter, H.. (1992); Morphologie und systematische Bedeutung des Pollens der Bromeliaceae. Grana. 31 197-212
27 Horres, R., and Zizka, G.. (1995); Untersuchungen zur Blattsukkulenz bei Bromeliaceae. Beitr. Biol. Pflanzen. 69 43-76
28 Linder, H. P., and Kellogg, E. A.. (1995) Phylogenetic patterns in the commelinid clade. Monocotyledons: Systematics and Evolution. Rudall, P. J., Cribb, P. J., Cutler, D. F., and Humphries, C. J., eds. Kew; Royal Botanic Gardens pp. 473-497
29 McDade, L. A., and Moody, M. L.. (1999); Phylogenetic relationships among Acanthaceae: Evidence from noncoding trnL-trnF chloroplast DNA sequences. Amer. J. Bot.. 86 70-80
30 Medina, E.. (1974); Dark CO₂ fixation, habitat preference and evolution within the Bromeliaceae. Evolution. 28 677-686
31 Mez, C.. (1934 - 1935) Bromeliaceae. Das Pflanzenreich, Vol. 4, 32. Engler, A., ed. Leipzig; W. Engelmann pp. 1-667
32 Ranker, T. A.,, Soltis, D. E.,, Soltis, P. S.,, and Gilmartin, A. J.. (1990); Subfamilial phylogenetic relationships of the Bromeliaceae: evidence from chloroplast DNA restriction site variation. Syst. Bot.. 15 425-434
33 Saitou, N., and Nei, M.. (1987); The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol.. 4 406-425
34 Sambrook, J.,, Fritsch, E. F.,, and Maniatis, T.. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor; Cold Spring Harbor Laboratory Press
35 Smith, L. B.. (1934); Geographical evidence on the lines of evolution in the Bromeliaceae. Bot. Jahrb.. 66 446-468
36 Smith, L. B., and Downs, R. J.. (1974 - 1979) Flora Neotropica 14, 1 - 3: Pitcairnioideae, Tillandsioideae, Bromelioideae. New York; Hafner Press (Vol. 1 + 2) New York; Bot. Gard. (Vol. 3)
37 Smith, L. B., and Till, W.. (1998) Bromeliaceae. The Families and Genera of Vascular Plants, Vol. 4. Kubitzki, K., ed. Berlin, Heidelberg, New York; Springer pp. 74-99
38 Spencer, M. A., and Smith, L. B.. (1992); A revision of the genus Deuterocohnia Mez (Bromeliaceae: Pitcairnioideae). Bradea. 16 141-146
39 Spencer, M. A., and Smith, L. B.. (1993); Racinaea, a new genus of Bromeliaceae (Tillandsioideae). Phytologia. 74 151-160
40 Swofford, D. L., and Begle, D. P.. (1993) PAUP. Phylogenetic Analysis Using Parsimony. Version 3.1. Champaign, Illinois; Smithsonian Institute
41 Taberlet, P.,, Gielly, L.,, Pautou, G.,, and Bouvet, J.. (1991); Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol.. 17 1105-1109
42 Terry, R. G.,, Brown, G. K.,, and Olmstead, R. G.. (1997 a); Examination of subfamilial phylogeny in Bromeliaceae using comparative sequencing of the plastid locus ndhF. Amer. J. Bot.. 84 664-670
43 Terry, R. G.,, Brown, G. K.,, and Olmstead, R. G.. (1997 b); Phylogenetic relationships in subfamily Tillandsioideae (Bromeliaceae) using ndhF sequences. Syst. Bot.. 22 333-345
44 Terry, R. G.,, Smith, J. A. C.,, Crayn, D.,, and Winter, K.. (1998); Reassessment of subfamily relationships in Bromeliaceae using noncoding sequences of the chloroplast genome. Amer. J. Bot.. 85 161-162 (Abstract)
45 Thompson, J. D.,, Gibson, T. J.,, Plewniak, F.,, Jeanmouguin, F.,, and Higgins, D. G.. (1997); The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res.. 25 4876-4882
46 Tomlinson, P. B.. (1969) Anatomy of the monocotyledons. III Commelinales - Zingiberales. Oxford; Clarendon
47 Varadarajan, G. S., and Gilmartin, A. J.. (1988 a); Seed morphology of subfamily Pitcairnioideae (Bromeliaceae) and its systematic implications. Amer. J. Bot.. 75 808-818
48 Varadarajan, G. S., and Gilmartin, A. J.. (1988 b); Phylogenetic relationships of groups of genera within the subfamily Pitcairnioideae (Bromeliaceae). Syst. Bot.. 13 283-293
49 Varadarajan, G. S., and Gilmartin, A. J.. (1988 c); Taxonomic realignments within the subfamily Pitcairnioideae (Bromeliaceae). Syst. Bot.. 13 294-299
50 Vos, P.,, Hogers, R.,, Bleeker, M.,, Reijans, M.,, Van de Lee, T.,, Hornes, M.,, Frijters, A.,, Pot, J.,, Peleman, J.,, Kuiper, M.,, and Zabeau, M.. (1995); AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res.. 23 4407-4414
51 Will, B., and Zizka, G.. (1999); The genus Greigia Regel in Chile. Harvard Papers in Botany. 4 225-240
52 Zizka, G.,, Horres, R.,, Nelson, E. C.,, and Weising, K.. (1999); Revision of the genus Fascicularia Mez (Bromeliaceae). Bot. J. Linn. Soc.. 129 315-332

G. Zizka

Botanisches Institut der Johann Wolfgang Goethe-Universität und Forschungsinstitut Senckenberg Senckenberganlage 25

60325 Frankfurt/M. Germany

eMail: gzizka@sngkw.uni-frankfurt.de

Section Editor: M. Hasebe