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DOI: 10.1055/s-2001-10629
© Georg Thieme Verlag Stuttgart · New York
The Inhibitory Effects of Ginsenosides on Protein Tyrosine Kinase Activated by Hypoxia/Reoxygenation in Cultured Human Umbilical Vein Endothelial Cells
Publikationsverlauf
February 29, 2000
June 18, 2000
Publikationsdatum:
31. Dezember 2001 (online)
![](https://www.thieme-connect.de/media/plantamedica/200101/lookinside/thumbnails/10.1055-s-2001-10629-1.jpg)
Abstract
27 individual ginsenosides and aglycones, together with five extracts from ginseng roots, ginseng leaves, American ginseng roots, American ginseng leaves and non-saponin fraction from roots of Panax ginseng, were tested for their effects on protein tyrosine kinase (PTK) activation induced by an in vitro hypoxia/reoxygenation (H/R) model in cultured human umbilical vein endothelial cells (HUVEC). The results indicated that ginsenoside-Rb1 (3), -Rd (7), -Ra1 (1) and -Ro (27) showed significant inhibitory effects on PTK activation induced by H/R. Dose-response experiments revealed that ginsenoside-Rb1 was the most active compound and it completely blocked PTK activation at a wide range of concentrations. Most protopanaxadiol-type ginsenosides and some protopanaxatriol-type saponins also showed significant effects on PTK activation. However, the crude extracts did not protect against H/R-induced PTK activation.
Key words
Panax ginseng - C. A. Meyer - Araliaceae - ginsenosides - hypoxia/reoxygenation - protein tyrosine kinase - tyrosine phosphorylated proteins
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Dr. De-Qiang Dou
Department of Natural Products Chemistry
Shenyang Pharmaceutical University
103 Wenhua Road
Shenyang 110015
P. R. China
eMail: doudeqiang@yahoo.com
Fax: 86-24-83890024