Planta Med 2001; 67(1): 19-23
DOI: 10.1055/s-2001-10629
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

The Inhibitory Effects of Ginsenosides on Protein Tyrosine Kinase Activated by Hypoxia/Reoxygenation in Cultured Human Umbilical Vein Endothelial Cells

De-Qiang Dou*, You-Wei Zhang, Lei Zhang, Ying-Jie Chen, Xin-Sheng Yao
  • Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang, China
Further Information

Publication History

February 29, 2000

June 18, 2000

Publication Date:
31 December 2001 (online)

Abstract

27 individual ginsenosides and aglycones, together with five extracts from ginseng roots, ginseng leaves, American ginseng roots, American ginseng leaves and non-saponin fraction from roots of Panax ginseng, were tested for their effects on protein tyrosine kinase (PTK) activation induced by an in vitro hypoxia/reoxygenation (H/R) model in cultured human umbilical vein endothelial cells (HUVEC). The results indicated that ginsenoside-Rb1 (3), -Rd (7), -Ra1 (1) and -Ro (27) showed significant inhibitory effects on PTK activation induced by H/R. Dose-response experiments revealed that ginsenoside-Rb1 was the most active compound and it completely blocked PTK activation at a wide range of concentrations. Most protopanaxadiol-type ginsenosides and some protopanaxatriol-type saponins also showed significant effects on PTK activation. However, the crude extracts did not protect against H/R-induced PTK activation.

References

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Dr. De-Qiang Dou

Department of Natural Products Chemistry

Shenyang Pharmaceutical University

103 Wenhua Road

Shenyang 110015

P. R. China

Email: doudeqiang@yahoo.com

Fax: 86-24-83890024