Cnidicin, a Coumarin, from the Root of Angelica koreana, Inhibits the Degranulation of Mast Cell and the NO Generation in RAW 264.7 Cells

Abstract

Cnidicin (1) and five related coumarins were isolated from the root extract of Angelica koreana (Umbelliferae) as active principles responsible for the inhibitory effect on the degranulation process of cultured mast cells. Cnidicin (1) demonstrated a significant inhibition upon the release of β-hexosaminidase from the cultured RBL-2H3 cells in a dose dependent manner (IC₅₀ value, 25 μM) and upon the nitric oxide production from the activated RAW264.7 cells (IC₅₀ value, 7.5 μM). In agreement with this, cnidicin inhibited the expression of nitric oxide synthase in RAW264.7 cells.

References

• 1 Choi S H, Kim Y R, Lim D K, Bae E O. Studies of anti-allergic actions of several natural products - I.  Yakhak Hoiji. 1992;  36 140-9
• 2 Choi O H, Kim J H, Kinet J P. Calcium mobilization via sphingosine kinase in signaling by the Fc epsilon RI antigen receptor.  Nature. 1996;  380 634-6
• 3 Fischer B, Schmutzler W. Inhibition by azelastine of the immunologically induced histamine release from isolated guinea pig mast cells.  Arzneimittelforschung. 1981;  31 1193-5
• 4 Yoshiyuki K, Hiromichi O. Histamine-release effectors from Angelica dahurica var. dahurica root.  J. Nat. Prod.. 1997;  60 249-51
• 5 Ohshima H, Bartsch H. Chronic infections and inflammatory processes as cancer risk factors: Possible role of nitric oxide in carcinogenesis.  Mutat. Res.. 1994;  305 253-64
• 6 Zingarelli B, Carnuccio R, Di Rosa M. Cloricromene inhibits the induction of nitric oxide synthase.  Eur. J. Pharmacol.. 1993;  243 107-11
• 7 Murakami A, Gao G, Omura M, Yano M, Ito C, Furukawa H, Takahashi D, Koshimizu K, Ohigashi H. 1,1-Dimethylallylcoumarins potently suppress both lipopolysaccharide- and interferon-gamma-induced nitric oxide generation in mouse macrophage RAW 264.7 cells.  Bioorg. Med. Chem. Lett.. 2000;  10 59-62
• 8 Walker G, Pfeilschifter J, Kunz D. Mechanisms of suppression of inducible nitric oxide synthase (iNOS) expression in interferon (IFN)-gamma-stimulated RAW 264.7 cells by dexamethasone. Evidence for glucocorticoid-induced degradation of iNOS protein by calpain as a key step in posttranscriptional regulation.  J. Biol. Chem.. 1997;  272 16679-87
• 9 Harkar S, Razdon T K, Waigh E S. Steroids, chromone and coumarins from Angelica officinalis .  Phytochemistry. 1984;  23 419-26
• 10 Cheong H, Ryu S Y, Kim K M. Anti-allergic action of resveratrol and related hydroxystilbenes.  Planta Med.. 1999;  65 266-8