Bakuchiol: A Hepatoprotective Compound of Psoralea corylifolia on Tacrine-Induced Cytotoxicity in Hep G2 Cells

Abstract

Bioassay-guided fractionation of the H₂O extract of the seeds of Psoralea corylifolia furnished one hepatoprotective compound, bakuchiol (1), together with two moderately active compounds, bakuchicin (2) and psoralen (3), on tacrine-induced cytotoxicity in human liver-derived Hep G2 cells. The EC₅₀ values of compounds 1 - 3 are 1.0, 47.0, 50.0 μg/ml, respectively. Silymarin as a positive control showed the EC₅₀ value with 5.0 μg/ml.

References

• 1 Watkins P B, Zimmerman H J, Knapp M J,. et al . Hepatotoxic effects of tacrine administration in patients with Alzheimer’s disease.  Journal of the American Medical Association. 1994;  271 992-8
• 2 Grant M H, Duthie S J, Gray A G,. et al . Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line.  Biochemical and Pharmacology. 1988;  37 4111-6
• 3 Viau C J, Curren R D, Wallace K. Cytotoxicity of tacrine and velnacrine metabolites in cultured rat, dog, and human hepatocytes.  Drug & Chemical Toxicology. 1993;  16 227-39
• 4 Zhu Y P. Chinese Materia Medica: Chemistry, Pharmacology and Applications. Amsterdam; Harwood Academic Publishers 1998: 609-12
• 5 Iwamura J, Dohi T, Tanaka H ,. et al . Cytotoxicity of Corylifoliae Fructus. II. Cytotoxicity of bakuchiol and the analogues.  Yakugaku Zasshi. 1989;  109 962-5
• 6 Buckingham J, editor. Dictionary of Natural Products. Vol. 2. London; Chapman & Hall 1994: 600-1
• 7 Kondo Y, Kato A, Kubota Y ,. et al . Bakuchicin, a new simple furanocoumarin from Psoralea corylifolia L.  Heterocycles. 1990;  31 187-90
• 8 Benoit G G, Naud C F, Simard M A,. et al . Noninterference of cytochrome P4501A2 in the cytotoxicity of tacrine using genetically engineered V79 Chinese hamster cells for stable expression of the human or rat isoform and two human hepatocyte cell lines.  Chemical Pharmacology. 1997;  53 423-7