DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification

Mareshige Kojoma; Osamu Iida; Yukiko Makino; Setsuko Sekita; Motoyoshi Satake

doi:10.1055/s-2002-19875

Planta Medica, Table of Contents

Planta Med 2002; 68(1): 60-63
DOI: 10.1055/s-2002-19875

Original Paper

Analysis
© Georg Thieme Verlag Stuttgart · New York

DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification

Mareshige Kojoma^1, ⁴ , Osamu Iida¹ , Yukiko Makino² , Setsuko Sekita³ , Motoyoshi Satake³

¹Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Japan
²Kanto-Shin'etsu Regional Narcotic Control Office, Japan
³Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences, Tokyo, Japan
⁴Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan

Abstract

Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L. for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could not be achieved by HPLC analysis.

Key words

Cannabis sativa - hemp - Cannabaceae - DNA profiling - ISSR - cannabinoid - HPLC

Full Text

References

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Mareshige Kojoma

Plant Functions Laboratory

RIKEN (The Institute of Physical and Chemical Research)

2-1 Hirosawa

Wako-shi

Saitama 351-0198

Japan

Fax: +81-48-467-5407

Email: kojoma@postman.riken.go.jp