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Planta Med 2002; 68(1): 60-63
DOI: 10.1055/s-2002-19875
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification

Mareshige Kojoma1, 4 , Osamu Iida1 , Yukiko Makino2 , Setsuko Sekita3 , Motoyoshi Satake3
  • 1Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Japan
  • 2Kanto-Shin'etsu Regional Narcotic Control Office, Japan
  • 3Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences, Tokyo, Japan
  • 4Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan
Further Information

Publication History

January 19, 2001

June 9, 2001

Publication Date:
31 January 2002 (online)

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Abstract

Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L. for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could not be achieved by HPLC analysis.

Key words

Cannabis sativa - hemp - Cannabaceae - DNA profiling - ISSR - cannabinoid - HPLC

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Mareshige Kojoma

Plant Functions Laboratory

RIKEN (The Institute of Physical and Chemical Research)


2-1 Hirosawa

Wako-shi

Saitama 351-0198

Japan

Fax: +81-48-467-5407

Email: kojoma@postman.riken.go.jp